Rhod-FF AM is a cell-permeant acetoxymethyl ester of the fluorescent calcium indicator rhod-FF.
Canonical SMILES | CN(C)C(C=C1)=CC2=C1C(C3=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC4=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=CC(F)=C4F)=C3)=C(C=C/5)C(O2)=CC5=[N+](C)\C.[Br-] |
分子式 | C51H55F2N4O19•Br |
分子量 | 1145.9 |
溶解度 | Soluble in DMSO |
储存条件 | Store at -20°C |
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. |
Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
产品描述 | Rhod-FF AM is a cell-permeant acetoxymethyl ester of the fluorescent calcium indicator rhod-FF . As rhod-FF AM enters cells, it is hydrolyzed by intracellular esterases to produce rhod-FF. Rhod-FF is a difluorinated analog of the cell-impermeant calcium indicator rhod-2. It has a very low affinity for calcium (Kd = 320 µM). Low affinity calcium indicators are particularly useful for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes will be insensitive to luminal fluctuations. Rhod-FF has excitation/emission maxima of approximately 552 and 580 nm, respectively. |