Cell lines

M. incognita, A. erubescens

Preparation method

The solubility of this compound in DMSO is >16.3mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

72 h

Applications

Schaftoside possessed strong nematicidal activity against M. incognita with the LC50 of 114.66 μg/mL. Schaftoside showed strong nematicidal activity against the crude extract of A. erubescens and exhibited nematicidal activity against the root-knot nematode with a LC50 value of 258.11 μg/mL.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

Schaftosideextracted from rice phloem can be usedas feeding inhibitors and resistance factors to brown planthoppers,Nilaparvatalugens[1].The screening of several Chinese medicinal herbs for nematicidal properties has shown thatArisaemaerubescens(Wall.) Schott tubers possessed significant nematicidal activity against the root-knot nematode (Meloidogyne incognita). Two nematicidal flavone-C-glycosides were isolated by bioassay-guided fractionation from the ethanol extract. on the basis of their phytochemical and spectral data, the compounds were identified as schaftoside and isoschaftoside. Schaftoside and isoschaftosideexihibited strong nematicidal activity againstM.incognitawith the LC50values of 114.66 μg/mL and 323.09 μg/mL, respectively, the crude extract ofA.erubescensexhibited nematicidal activity against the root-knot nematode with a LC50value of 258.11 μg/mL[2].

References:
[1]. Stevenson P C, Kimmins F M, Grayer R J, et al. Schaftosides from rice phloem as feeding inhibitors and resistance factors to brown planthoppers, Nilaparvatalugens[J]. EntomologiaExperimentalisetApplicata, 1996, 80(1): 246-249.
[2]. Du S S, Zhang H M, Bai C Q, et al. Nematocidal flavone-C-glycosides against the root-knot nematode (Meloidogyne incognita) from Arisaemaerubescens tubers[J]. Molecules, 2011, 16(6): 5079-5086.