Dinactin

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Dinactin

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

20261-85-2

包装与价格：

包装	价格(元)
1mg	询价
5mg	询价

化学性质

Physical Appearance	A film
Storage	Store at -20°C
M.Wt	765
Cas No.	20261-85-2
Formula	C₄₂H₆₈O₁₂
Solubility	Soluble in DMSO
Chemical Name	(1R,2R,5R,7R,10S,11S,14S,16S,19R,20R,23R,25R,28S,29S,32S,34S)-5,23-diethyl-2,11,14,20,29,32-hexamethyl-4,13,22,31,37,38,39,40-octaoxapentacyclo[32.2.1.1^7,10.1^16,19.1^25,28]tetracontane-3,12,21,30-tetrone
Canonical SMILES	O=C(O[C@H](CC)C[C@]1([H])O[C@@]([C@@H]2C)([H])CC1)[C@H](C)[C@@]3([H])CC[C@](C[C@H](C)OC([C@H]([C@@]4([H])O[C@](C[C@H](OC([C@H](C)[C@]5([H])O[C@](CC5)([H])C[C@H](C)OC2=O)=O)CC)([H])CC4)C)=O)([H])O3
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Dinactin is a macrotetrolide antibiotic that acts as an ionophore for monovalent cations, such as NH4+, K+, and Rb+. Dinactin has been used to study the membrane properties [1]. Dinactin is considered as an active molecule that S. araujoniae produced to control B. cinerea[2].

In vitro: Dinactin inhibited T-cell proliferation induced by IL-2, by mAb to CD3, and by mAbs to CD3 plus α-CD28 with identical dose-response curves. The IC50 was 10–20 ng/ml. Dinactin inhibited cytokine production with IC50 values of 10 ng/ml for IL-4 and IL-5, 30 or 60 ng/ml for interferong or IL-2, respectively [3].Dinactin inhibited cytokine production through a post-transcriptional mechanism. Dinactin also reduced pulmonary eosinophilia when administered within 1 d of airway antigen challenge [3].

References:
[1] Laprade R, Grenier F, Pagé-Dansereau M, et al. Carrier-mediated ion transport in lipid bilayer membranes[J]. Canadian journal of biochemistry and cell biology, 1984, 62(8): 738-751.
[2] Silva L J, Crevelin E J, Souza W R, et al. Streptomyces araujoniae produces a multiantibiotic complex with ionophoric properties to control Botrytis cinerea[J]. Phytopathology, 2014, 104(12): 1298-1305.
[3] Umland S P, Shah H, Jakway J P, et al. Effects of cyclosporin A and dinactin on T-cell proliferation, interleukin-5 production, and murine pulmonary inflammation[J]. American journal of respiratory cell and molecular biology, 1999, 20(3): 481-492.

试验操作

细胞实验 [1]：
细胞系	CD4⁺细胞
溶解方法	在DMSO中是可溶的。为了获得更高的浓度，可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。
反应条件	10～1000ng/ml
应用	Dinactin和monactin对IL-5的产生具有类似的抑制作用（IC50 = 20 ng / ml），并且在MTS实验中中对细胞生存力表现出微弱的抑制作用（1,000 ng / ml时抑制30％）。相反，nonactin是抑制IL-5产生的较弱抑制剂（IC50 = 200 ng / ml）。CSA是α-CD3 mAb刺激SP-B21细胞产生IL-5的有效抑制剂（IC50 = 50 ng / ml）；在100 ng / ml处观察到完全抑制，没有毒性证据。
References: [1]. Umland SP, Shah H, Jakway JP, Shortall J, Razac S, Garlisi CG, Falcone A, Kung TT, Stelts D, Hegde V, Patel M, Motasim Billah M, Egan RW. Effects of cyclosporin A and dinactin on T-cell proliferation, interleukin-5 production, and murine pulmonary inflammation. Am J Respir Cell Mol Biol. 1999 Mar;20(3):481-92. PubMed PMID: 10030847.