Caspase-6 Colorimetric Assay Kit

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包装与价格：

包装	价格(元)
25 assays	询价
100 assays	询价
200 assays	询价
400 assays	询价

组份和存储

Components	K2015-25 25 assays	K2015-100 100 assays	K2015-200 200 assays	K2015-400 400 assays	Part Number
Cell Lysis Buffer	25 ml	100 ml	100 ml	100 ml	K2015-C-1
2 X Reaction Buffer	2 ml	4 x 2 ml	16 ml	32 ml	K2015-C-2
VEID-pNA (4 mM)	125 μl	500 μl	2 x 0.5 ml	2 x 1 ml	K2015-C-3
DTT (1 M)	100 μl	400 μl	400 μl	400 μl	K2015-C-4
Store at -20°C.

资料参考

Cysteine-dependent aspartate-directed proteases (Caspases) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Sequential activation of caspases plays an important role in cell apoptosis. Caspase-6 is a member of the caspase family. Caspase-6 is processed by caspases 7, 8 and 10, and can also undergo self-processing. Caspase-6 acts as a downstream enzyme in the caspase activation cascade and interacts with Caspase 8.

Caspase-6 Colorimetric Assay Kit provides a convenient and simple way for detecting the VEID-dependent caspase activity. When cleavage of VEID from the labeled substrate VEID-p-nitroaniline (VEID-pNA) by Caspase-6 or related caspases, the free pNA light emission can be quantified by using a microtiter plate reader or a spectrophotometer at 405 or 400 nm. Comparison of the absorbance of free pNA from an apoptotic sample with an uninduced control determines the fold increase in Caspase-6 activity.

Features & Properties

Features	Fast and convenient Simple one-step procedure; takes 1-2 hours. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-6 activity.
Shipping	Gel pack
Storage Conditions	Store at -20°C.

概述

Species reactivity	Mammalian
Applications	Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Kit components	Cell Lysis Buffer 2X Reaction Buffer VEID-pNA (4 mM) DTT (1 M)