Cell experiment:

Detection of apoptosis and necrosis is analyzed by Annexin V and propidium iodide staining. After incubation in the absence or in the presence of 8-Azaguanine, cells are washed (centrifuged at 300×g during 5 min) and incubated for 10 min at 4℃ in 440 μL Annexin buffer, containing 5 μL Annexin V and 5 μL propidium iodide. Then cells are washed and re-suspended in phosphate buffered saline (PBS) until analysis[1].

8-Azaguanine is a purine analogue which shows antineoplastic activity.

8-Azaguanine is a purine analogue which shows antineoplastic activity. The results show that cell viability decreases after incubation with 8-Azaguanine, in a dose and time dependent manner. After 24 h of treatment with 8-Azaguanine the IC50 for CEM cell line is about 100 μM while for MOLT3 cell line the IC50 is about 10 μM. CEM and MOLT3 cells surface expression of CD26 increases after treatment with 8-Azaguanine in a concentrations dependent manner[1].

References:
[1]. Dourado M, et al. CD26/DPPIV expression and 8-azaguanine response in T-acute lymphoblastic leukaemia cell lines in culture. Pathophysiology. 2007 May;14(1):3-10.