5-Ethynyl-2'-deoxyuridine(5-EdU)

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CAS NO:

61135-33-9

包装与价格：

包装	价格(元)
100mg	询价
1g	询价

化学性质

Physical Appearance	Solution
Storage	Store at -20°C
M.Wt	252.22
Cas No.	61135-33-9
Formula	C₁₁H₁₂N₂O₅
Solubility	≥25.2 mg/mL in DMSO; insoluble in EtOH; ≥3.16 mg/mL in H2O with gentle warming and ultrasonic
Chemical Name	5-ethynyl-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidine-2,4(1H,3H)-dione
Canonical SMILES	O=C1C(C#C)=CN([C@@H]2O[C@H](CO)[C@@H](O)C2)C(N1)=O
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

描述

5-Ethynyl-2'-deoxyuridine (5-EdU) is a thymidine analog that can be incorporated into replicating DNA for detection of cell proliferation. 5-EdU can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle through click chemistry. Click chemistry is a method of covalently coupling an azide with an alkyne. 5-EdU has an alkyne handle that can be detected using azide-containing fluorescent probes through a highly efficient click chemistry reaction [1][2]. In contrast to the use of BrdU, the new method does not require sample fixation or DNA denaturation. 5-EdU-labelled cells may be isolated to determine the transcript of cells, from neural cancer or tissues that have undergone division in vitro and in vivo[3].

References：

[1] Zeng C, Pan F, et al. Evaluation of 5-ethynyl-2'-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system. Brain Res. 2010 Mar 10;1319:21-32.

[2] Cavanagh BL, Walker T, et al. Thymidine analogues for tracking DNA synthesis. Molecules. 2011 Sep 15;16(9):7980-93.

[3] Endaya BB, Lam PY, et al. Transcriptional profiling of dividing tumor cells detects intratumor heterogeneity linked to cell proliferation in a brain tumor model. Mol Oncol. 2016 Jan;10(1):126-37.