化学性质
| Physical Appearance | A solid |
| Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| M.Wt | 601.22 |
| Formula | C35H45ClN6O |
| Solubility | ≥60.1 mg/mL in DMSO; insoluble in H2O; ≥49.1 mg/mL in EtOH with ultrasonic |
| Chemical Name | 1-(6-((3-azidopropyl)amino)-6-oxohexyl)-3,3-dimethyl-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-3H-indol-1-ium chloride |
| Canonical SMILES | CN1C2=CC=CC=C2C(C)(C)/C1=C\C=C\C=C\C3=[N+](CCCCCC(NCCCN=[N+]=[N-])=O)C(C=CC=C4)=C4C3(C)C.[Cl-] |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
资料参考
Cy5 azide is a labeling reagent ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. Alkyne-modified biomolecules in mixtures of water with organic solvents can be labeled by Cy5 azide.
Cy5/BHQ2, which is widely used in the research, combinations on several TaqMan probes with various degree of stability of secondary structures and provides some advice on the applicability of dye/quencher pairs for DNA dual labeled probes [1].
Reference:
[1] Farzan, V. M.; Aparin, I.O.; Veselova, O.A.; Podkolzin, A.T.; Shipulin, G.A.; Korshun, V.A.; Zatsepin, T.S. Cy5/BHQ dye-quencher pairs in fluorogenic qPCR probes: effects of charge and hydrophobicity. Analytical Methods, in press.
Spectral properties
| 最大激发光波 (nm) | 646 | 最大发射光波 (nm) | 662 |
| 消光系数 (M-1cm-1) | 250000 | 量子产量 | 0.2 |
| CF260 | 0.03 | CF280 | 0.04 |
