化学性质
Physical Appearance | A solid |
Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
M.Wt | 717.38 |
Formula | C44H53ClN6O |
Solubility | ≥71.7 mg/mL in DMSO; insoluble in H2O; ≥44.2 mg/mL in EtOH with ultrasonic |
Chemical Name | 3-(6-((3-azidopropyl)amino)-6-oxohexyl)-2-((1E,3E)-3-(3-butyl-1,1-dimethyl-1H-benzo[e]indol-2(3H)-ylidene)prop-1-en-1-yl)-1,1-dimethyl-1H-benzo[e]indol-3-ium |
Canonical SMILES | CC1(C)C2=C3C(C=CC=C3)=CC=C2[N+](CCCCCC(NCCCN=[N+]=[N-])=O)=C1/C=C/C=C4C(C)(C)C5=C(C=CC=C6)C6=CC=C5N\4CCCC |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
资料参考
Cy3.5 azide is a solid material dye ready for the use in Click Chemistry reaction. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.
Cy3.5 azide could easily interfere with a radical polymerisation process as it contains a variety of double bonds. The Cy-3 modified initiator was then used to grow the modified PMPC-PDPA directly from the dye [1].
Reference:
[1] Gaitzsch, J. ; Delahaye, M.; Poma, A.; Du Prez, F.; Battaglia, G. Comparison of metal free polymer-dye conjugation strategies in protic solvents. Polymer Chemistry, 2016, 7(17), 3046–3055.
Spectral properties
最大激发光波 (nm) | 591 | 最大发射光波 (nm) | 604 |
消光系数 (M-1cm-1) | 116000 | 量子产量 | 0.35 |
CF260 | 0.29 | CF280 | 0.22 |