Nile Red

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Nile Red

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

7385-67-3

包装与价格：

包装	价格(元)
10mg	询价
50mg	询价
100mg	询价
500mg	询价

化学性质

Storage	Store at -20°C
M.Wt	318.37
Cas No.	7385-67-3
Formula	C₂₀H₁₈N₂O₂
Solubility	≥2.56 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O
Chemical Name	9-(diethylamino)-5H-benzo[a]phenoxazin-5-one
Canonical SMILES	CCN(C1=CC2=C(N=C3C4=CC=CC=C4C(C=C3O2)=O)C=C1)CC
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Nile Red, is a fluorescent stain specific for the detection of intracellular lipid droplets in hydrophobic environment, but show minimal fluorescence in aqueous media. Nile Red can be applied for staining intracellular lipids, lysosomal phospholipid inclusions and hydrophobic domains of proteins, and detected by fluorescence microscopy and flow cytofluorometry. In all organic solvents, Nile Red is intensely fluorescent, with colors ranging from golden yellow to red.

Reference:

1. Greenspan P, Mayer EP, Fowler SD. Nile red: a selective fluorescent stain for intracellular lipid droplets. Journal of Cell Biology, 1985, 100(3): 965-973.

试验操作

Cell experiment:[1]
Cell lines	Monkey aortic smooth muscle cells and mouse peritoneal macrophages, induced by acetylated low density lipoprotein
Reaction Conditions	100 ng/ml Nile Red
Applications	Better selectivity for cytoplasmic lipid droplets was obtained when the cells were viewed for yellow-gold fluorescence (excitation, 450-500 nm; emission, greater than 528 nm) rather than red fluorescence (excitation, 515-560 nm; emission, greater than 590 nm).
Note	The technical data provided above is for reference only.
	References: 1. Greenspan P, Mayer EP, Fowler SD. Nile red: a selective fluorescent stain for intracellular lipid droplets. Journal of Cell Biology, 1985, 100(3): 965-973.