描述
| Gene ID: | 834 | Accession #: | NP_150634.1 |
| Alternate Names: | Active Human Caspase-1, Active Caspase-1, Caspase-1 Enzyme, Recombinant caspase-1, human caspase-1, human caspases, active caspases. |
| Source: | Escherichia Coli. | M.Wt: | large (20 kD) and small (10 kD) subunits |
| Appearance: | Semi-Dry powder | Storage: | Store at -70℃ |
| Formulation: | Lyophilized powder |
| Solubility: | Reconstitute to 1 unit per μl in PBS containing 15% glycerol. |
| Shipping Condition: | gel pack. | Handling: | Centrifuge the vial prior to opening. |
| Usage: | For Research Use Only! Not to be used in humans. |
资料参考
Caspase-1 (also know as Interleukin-1β-Converting Enzyme) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme, which is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the mechanism of regulation of caspase-1 activation is complex and poorly understood.
The recombinant active human caspase-1 was expressed in E. coli. The active caspase-1 preferentially cleaves caspase-1 substrates (YVAD-AFC or YVAD-pNA) and is routinely tested for its ability to enzymatically cleave Ac-YVAD-pNA or Ac-YVAD-AFC. The recombinant human Caspase-1 has an N-term His-tag and corresponds to amino acids 120-404 of Caspase-1.
Active caspase-1 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.生物活性
| Specific activity | > 5000 units/mg. |
