资料参考
Cysteine-dependent aspartate-directed proteases (Caspases) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Sequential activation of caspases plays an important role in cell apoptosis. Caspase-8 is a caspase protein that cleaves and activates Caspase-3 and is involved in the programmed cell death induced by Fas and different apoptotic stimuli. Caspase-8 was detected in the insoluble fraction of the affected brain region in Huntington disease patients, which suggested it plays an important role in neurodegenerative diseases.
Caspase-8 Fluorometric Assay Kit provides a convenient and simple way for detecting the IETD-dependent caspase activity. IETD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin) emits blue light (λmax = 400 nm); while cleavage of IETD-AFC by Caspase-8 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified by using a fluorecence microtiter plate reader or a fluorometer. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control determines the fold increase in Caspase-8 activity.
Features & Properties
| Features | Fast and convenient
Simple one-step procedure; takes 1-2 hours.
Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-8 activity. |
| Shipping | Gel pack |
| Storage Conditions | Store at -20°C. |
概述
| Species reactivity | Mammalian |
| Applications | Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. |
| Kit components | Cell Lysis Buffer
2X Reaction Buffer
IETD-AFC (1mM)
DTT (1 M) |
