化学性质
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 653.77 |
| Formula | C38H54Cl2N4O |
| Solubility | ≥48 mg/mL in DMSO; insoluble in H2O; ≥8.84 mg/mL in EtOH |
| Chemical Name | 6-[6-[(2E)-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethylindol-1-ium-2-yl)penta-2,4-dienylidene]indol-1-yl]hexanoylamino]hexylazanium |
| Canonical SMILES | CC1(C2=CC=CC=C2[N+](=C1C=CC=CC=C3C(C4=CC=CC=C4N3CCCCCC(=O)NCCCCCC[NH3+])(C)C)C)C |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
资料参考
Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents. For instance, this amine can be coupled with EDC-activated carboxylic groups. With this bright and photostable reagent, many different methods of fluorescence detection is available and colorful fluorophore can also be easily detected in small quantities by naked human eye. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.
In A2780 cells, the conjugates polymer 2P-Cy3-Cy5 can be used for the analysis of the pair-correlation functions between Cy3 and Cy5. Treated with 2P-Cy3-Cy5 for several hours show similar shape and characteristic decay lengths [1].
Reference:
[1]Hartley, J. M.; Zhang, R.; Gudheti, M.; Yang, J.; Kopeek, J. Tracking and quantifying polymer therapeutic distribution on a cellular level using 3D dSTORM. Journal of Controlled Release, 2016, 231, 50–59.
Spectral properties
| 最大激发光波 (nm) | 646 | 最大发射光波 (nm) | 662 |
| 消光系数 (M-1cm-1) | 250000 | 量子产量 | 0.2 |
| CF260 | 0.03 | CF280 | 0.04 |
