NHC-diphosphate

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NHC-diphosphate

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CAS NO:

39023-73-9

NHC-diphosphate 是 NHC (HY-125033) 的活性胞内磷酸化代谢物 (intracellular metabolite)，以二磷酸盐的形式存在。NHC 是一种嘧啶核糖核苷，是一种有效的抗病毒剂 (anti-virus agent)。NHC 有效抑制委内瑞拉马脑炎病毒 (VEEV)、基孔肯亚病毒 (CHIKV) 和丙型肝炎病毒 (HCV) 的复制。

生物活性

NHC-diphosphate is an active phosphorylated intracellularmetabolite of β-d-N4-Hydroxycytidine (NHC) (HY-125033) as a diphosphate form^[1]. NHC is a pyrimidine ribonucleoside and behaves as a potentanti-virus agent. NHC effectively inhibits the replication ofvenezuelan equine encephalitisvirus (VEEV),Chikungunyavirus (CHIKV) andhepatitisC virus (HCV)^[1].

体外研究
(In Vitro)

Huh-7 cells are incubated with (10-50 μM; 4 h) NHC or a McGuigan phosphoramidate prodrug of NHC.
Intracellular levels of the parental compounds and phosphorylated metabolites are measured using LC-MS/MS. Small amounts of NHC-monophosphate (MP) and NHC-diphosphate (DP; HY-135867D) can be observed, while NHC-triphosphate remains the most abundant metabolite.^[1].

分子量

419.18

性状

Solid

Formula

C₉H₁₅N₃O₁₂P₂

CAS 号

39023-73-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

溶解性数据

In Vitro:

H₂O : 160 mg/mL(381.70 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	2.3856 mL	11.9281 mL	23.8561 mL
5 mM	0.4771 mL	2.3856 mL	4.7712 mL
10 mM	0.2386 mL	1.1928 mL	2.3856 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light, stored under nitrogen)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。