Metronidazole hydrochloride (SC 326421) 是一种具有口服活性的硝基咪唑类抗生素，能用于研究厌氧微生物的感染。Metronidazole hydrochloride 能够穿过血脑屏障，长期使用会导致炎症和骨骼肌收缩。
| 生物活性 | Metronidazole hydrochloride (SC 326421) is an orally active nitroimidazoleantibiotic, can be used to research anaerobic infections. Metronidazole hydrochloride can cross blood brain barrier and results inflammation and skeletal muscle contraction under long-term application[1][2][3][4]. |
体外研究
(In Vitro) | Metronidazole hydrochloride acts on anaerobic microorganism following: passage through the cell membrane; (b) reductive action; (c) interaction with intracellular targets; (d) release of inactive end products, exerts function with toxic nitro and nitroso derivatives[1].
Metronidazole hydrochloride displays inhibitory activity towards anaerobic protozoaTrichomonas vaginalis,Entamoeba histolytica,Giardia lamblia, andBalantidium coli[1].
Metronidazole hydrochloride (4-8 μg/mL) inhibits anaerobic bacteria and shows good bactericidal activity[1].
Metronidazole hydrochloride (0.1 μg/mL-0.01 mg/mL; 12-96 h) induces granular formation and triggers apoptosis inBlastocystissp[2].
Apoptosis Analysis[2] | Cell Line: | Blastocystissp. Cells | | Concentration: | 0.1 μg/mL-0.01 mg/mL | | Incubation Time: | 12, 24, 48, 60, 72, 84, 96 hours | | Result: | Decreased cell diameter, as a hallmark of an apoptotic cell, and resulted cell shrinkage. |
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体内研究
(In Vivo) | Metronidazole hydrochloride (135 mg/kg/d; p.o.; 28 d) can cross the blood brain barrier, and exhibits neurotoxicity under long term administration in rats[3].
Metronidazole hydrochloride (1 g/L; p.o.; 4 weeks) results skeletal muscle atrophy and changes the expression of genes involved in the muscle peripheral circadian rhythm machinery and metabolic regulation[4].
| Animal Model: | Sprague-Dawley (SD) rats (200-220 g)[3] | | Dosage: | 135 mg/kg | | Administration: | Oral gavage; once daily; 28 days | | Result: | Caused inflammatory markers increasing, including iNOS, eNOS, Bax and caspase 3 protein expressions increasing and caused oxidative stress damage in brain tissue, with MDA content rising. |
| Animal Model: | SPF C57Bl/6J mice (6-7 months old)[4] | | Dosage: | 1 g/L (full dose) | | Administration: | Oral gavage; provided with drinking water for 4 weeks, changed twice weekly | | Result: | Resulted the muscle core clock and effector genes Cry2, Ror-β, E4BP4, PP ARγ and adiponectin expression increasing.
Decreased hind limb muscle weight and resulted in smaller fibers in the tibialis anterior muscle. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
