Mirin

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Mirin

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

299953-00-7

Mirin 一种有效的Mre11-Rad50-Nbs1 (MRN)复合物抑制剂。Mirin 在不影响 ATM 蛋白激酶活性的情况下阻止 ATM (ataxia-telangiectasia mutated) 的 MRN 依赖性激活 (IC₅₀=12 μM)，并抑制 Mre11 相关的核酸外切酶活性。Mirin 可消除哺乳动物细胞中的 G2/M 检查点和同源性依赖性修复。Mirin 在哺乳动物细胞中阻止 ATM 因 DNA 双链断裂 (DSBs) 而激活并阻断同源性定向修复 (HDR)。

生物活性

Mirin is a potentMre11-Rad50-Nbs1 (MRN) complexinhibitor. Mirin prevents MRN-dependent activation ofATM(IC₅₀=12 μM) without affectingATMprotein kinase activity, and it inhibits Mre11-associated exonuclease activity. Mirin abolishes the G2/M checkpoint and homology-dependent repair in mammalian cells. Mirin preventsATMactivation in response to DNA double-strand breaks (DSBs) and blocks homology-directed repair (HDR) in mammalian cells^[1].

体外研究
(In Vitro)

Mirin inhibits H2AX phosphorylation with an IC₅₀of 66 μM. Mirin also inhibits the ATM-dependent phosphorylation of the downstream targets Nbs1 and Chk2 and the MRN-dependent autophosphorylation of ATM at Ser1981 in response to DSBs. Mirin induces a substantial G2 arrest at concentrations of 50 μM and 100 μM. Mirin (10-100 μM) inhibits homology-dependent DNA repair in TOSA4 cells^[1].
BRCA2-deficient cells also showed hypersensitivity to the Mre11 inhibitor Mirin^[2].

分子量

220.25

性状

Solid

Formula

C₁₀H₈N₂O₂S

CAS 号

299953-00-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据

In Vitro:

DMSO : 83.33 mg/mL(378.34 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	4.5403 mL	22.7015 mL	45.4030 mL
5 mM	0.9081 mL	4.5403 mL	9.0806 mL
10 mM	0.4540 mL	2.2701 mL	4.5403 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。