Pyronin Y (Pyronine G) 是一种可插入 RNA 的阳离子染料，可以靶向包括 RNA，DNA 和细胞器的细胞结构。Pyronin Y 与双链核酸(尤其是 RNA) 形成荧光复合物，可以对细胞 RNA 进行半定量分析。Pyronin Y 可用于鉴定活细胞中核糖核蛋白复合物的特定 RNA 亚种。
| 生物活性 | Pyronin Y (Pyronine G) is a cationic dye that intercalates RNA and has been used to target cell structures including RNA, DNA and organelles. Pyronin Y forms fluorescent complexes with double-stranded nucleic acids (especially RNA) enabling semi-quantitative analysis of cellular RNA. Pyronin Y can be used to identify specific RNA subspecies of ribonuclear proteins complexes in live cells[1][2][5]. |
体外研究
(In Vitro) | Pyronin Y forms fluorescent complexes with double-stranded nucleic acids, especially RNA, enabling semi-quantitative analysis of cellular RNA in flow cytometry, to estimate the RNA content per cell in formalin fixed EL4 leukosis tumor cells, enzyme dispersed R3327-G rat prostatic adenocarcinoma cells, mouse spleen cells stimulated with concanavalin A, and human peripheral blood lymphocytes stimulated with phytohemagglutinin[1].
A fluorescent staining procedure based on pyronin Y is described. The technique has been used to stain RNA in human reticulocytes for subsequent flow analysis and sorting[2].
In viable cells this dye also accumulates in mitochondria. At a concentration of 1.7 to 3.3 μM, pyronin Y is localized almost exclusively in mitochondria of cultured cells, similar to another mitochondria! probe, rhodamine 123. At that concentration PY is not toxic but suppressed cell growth, arresting cells[3].
Pyronin Y has long been used, in combination with other dyes such as Methyl Green, as a differential stain for nucleic acids in paraffin tissue sections. In sections stained with Methyl Green-pyronin Y, red blood cells, elastic fibre of blood vessels, zymogen granules of pancreatic acinar cells, surface membrane of heptocytes and kidney tubular cells showed strikingly strong green and/or red fluorescence, while the nuclei of cells appeared non-fluorescent[4].
Pyronin Y binds to double stranded RNA (dsRNA) including messenger RNA (mRNA), transfer RNA (tRNA) and ribosomal RNA (rRNA)[5].
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| 分子量 | |
| 性状 | |
| Formula | |
| CAS 号 | |
| Emission (Em) | Em 570-590 nm Yellow |
| Excitation (Ex) | Ex 495-570 nm Green |
| 中文名称 | |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | -20°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
| 溶解性数据 | In Vitro: DMSO : 25 mg/mL(82.56 mM;Need ultrasonic) H2O : 4 mg/mL(13.21 mM;Need ultrasonic) 配制储备液 | 1 mM | 3.3025 mL | 16.5126 mL | 33.0251 mL | | 5 mM | 0.6605 mL | 3.3025 mL | 6.6050 mL | | 10 mM | 0.3303 mL | 1.6513 mL | 3.3025 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 |
| 染色示例 | Description: Pyronin Y is a nucleic acid-specific dye preferably recognizing RNA.Method: For cell staining.1. Cells are fixed with 4% paraformaldehyde at 4℃.2. Stain cells with Pyronin Y.3. Confocal imaging is performed at the plane of nuclei, confocal microscopy images are taken on the Leica TCS SP8 WLL system.
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