Pyridostatin hydrochloride

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本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

1781882-65-2

RR82 hydrochloride

Pyridostatin (RR82) hydrochloride 是一种高选择性的G-quadruplex DNA稳定剂 (K_d=490 nM)。Pyridostatin hydrochloride 通过诱导复制和转录依赖的 DNA 损伤促进人类癌细胞生长停滞。Pyridostatin hydrochloride 靶向原癌基因 Src。Pyridostatin hydrochloride 降低人乳腺癌细胞 SRC 蛋白水平和 SRC 依赖的细胞运动。

生物活性

Pyridostatin (RR82) hydrochloride is aG-quadruplexDNAstabilizing agent (K_d=490 nM). Pyridostatin hydrochloride promotes growth arrest in humancancercells by inducing replication- and transcription-dependent DNA damage. Pyridostatin hydrochloride targets the proto-oncogeneSrc. Pyridostatin hydrochloride reducedSRCprotein levels and SRC-dependent cellular motility in human breastcancercells^[1]^[2].

IC₅₀& Target

Kd: 490 nM (G-quadruplexe)^[1]

体外研究
(In Vitro)

Pyridostatin (RR82) hydrochloride (10 μM; 48 hours) induces cell cycle arrest^[1].
Pyridostatin hydrochloride is a very selective G-quadruplex DNA-binding small molecule designed to form a complex with and stabilize G-quadruplex structure. Pyridostatin hydrochloride causes neurite retraction, synaptic loss, and dose-dependent neuronal death. In cultured primary neurons, Pyridostatin hydrochloride induces the formation of DNA DSBs. Remarkably, Pyridostatin hydrochloride (1-5 μM, overnight) downregulates the BRCA1 protein, a protein that guards and repairs the neuronal genome, at the transcriptional level^[3].

Cell Viability Assay^[1]

Cell Line:	Over 60 different cancer cell lines
Concentration:	10 μM
Incubation Time:	48 hours
Result:	Predominantly accumulated in the G2 phase of the cell cycle over 60 different cancer cell lines.

分子量

778.94

性状

Solid

Formula

C₃₁H₃₇Cl₅N₈O₅

CAS 号

1781882-65-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

溶解性数据

In Vitro:

H₂O : 50 mg/mL(64.19 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	1.2838 mL	6.4190 mL	12.8380 mL
5 mM	0.2568 mL	1.2838 mL	2.5676 mL
10 mM	0.1284 mL	0.6419 mL	1.2838 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (stored under nitrogen)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (128.38 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。