IXA6 是一种新型的IRE1/XBP1s激动剂，可诱导 IRE1 核糖核酸酶活性。
| 生物活性 | IXA6 is a novelIRE1/XBP1sactivator, and can induceIRE1RNase activity[1]. |
体外研究
(In Vitro) | IXA6 (10 μM; 4 or 18 h) selectively activates IRE1-XBP1s signaling, and activates the XBP1s transcriptional response[1].
IXA6 (10 μM; 4 h) shows selectivity for IRE1-XBP1s-dependent ER proteostasis remodeling[1].
IXA6 (10 μM; 18 h) reduces secretion of APP through IRE1 activation[1].
Western Blot Analysis[1] | Cell Line: | HEK293T cells | | Concentration: | 10 μM | | Incubation Time: | 18 hours | | Result: | Increasesd in ER proteostasis factor gene expression correspond to increased protein levels. |
RT-PCR[1] | Cell Line: | HEK293T cells | | Concentration: | 10 μM | | Incubation Time: | 4 hours | | Result: | Activated the IRE1-XBP1s geneset to levels about 30-40% that observed for Tg (Tg representing 100% activation of each gene). |
Cell Viability Assay[1] | Cell Line: | HEK293T cells | | Concentration: | 10 μM | | Incubation Time: | 4 hours | | Result: | Observed high level of overlap (64%) for genes induced by XBP1s and IXA6. |
RT-PCR[1] | Cell Line: | Huh7 and SHSY5Y cells | | Concentration: | 10 μM | | Incubation Time: | 4 hours | | Result: | Upregulated XBP1s mRNA selectively, in cell lines including Huh7 and SHSY5Y cells. |
RT-PCR[1] | Cell Line: | CHO cells | | Concentration: | 10 μM | | Incubation Time: | 18 hours | | Result: | Observed reduction in Aβ secretion blocked by cotreatment with 4μ8c, confirming this reduction is dependent on IRE1 RNAse activity in CHO7WD10 cells stably expressing wild-type APP (APPWT) and in cells treated with the IXA6. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
