Cytochalasin D

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Cytochalasin D

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

22144-77-0

细胞松弛素D
Zygosporin A
NSC 209835

Cytochalasin D (Zygosporin A) 是一种有效的肌动蛋白聚合抑制剂，可以来源于真菌。Cytochalasin D 具有细胞渗透活性。Cytochalasin D 通过结合 G-actin 抑制 G-actin–cofilin 的相互作用。Cytochalasin D 还能抑制 cofilin 与 F-actin 的结合，降低了活细胞中肌动蛋白聚合和解聚的速率。Cytochalasin D 可以减少外泌体的释放，进而减少肿瘤环境中 survivin 的量。 Cytochalasin D 诱导 Yap 磷酸化，阻止其核易位保留在胞质中。

生物活性

Cytochalasin D (Zygosporin A) is a potentactin polymerizationinhibitor, could be derived from fungus. Cytochalasin D has cell-permeable activity. Cytochalasin D inhibits the G-actin–cofilin interaction by binding to G-actin. Cytochalasin D also inhibits the binding of cofilin to F-actin and decreases the rate of both actin polymerization and depolymerization in living cells. Cytochalasin D can reduce exosome release, in turn reducing the amount ofsurvivinpresent in the tumour environment. Cytochalasin D induces phosphorylation and cytoplasmic retention ofYap^[1]^[2]^[3]^[4].

体外研究
(In Vitro)

Cytochalasin D (3 and 10 μM; 30 min) causes retraction and arborization of COS-7 cells and conversion of F-actin from long fibers to punctate structures^[1].
Cytochalasin D (0.3, 1, 3 and 10 μM; 30 min) concentration-dependently decreases the rate of actin depolymerization in COS-7 cells^[1].
Cytochalasin D (1 μM; NIH3T3 cells) disrupts stress fibers, induces phosphorylation of Yap, while cells maintains original area and lost nuclear Yap localization^[4].

Western Blot Analysis

Cell Line:	COS-7 cells^[1]
Concentration:	3 and 10 μM
Incubation Time:	30 min
Result:	Caused retraction and arborization of COS-7 cells and conversion of F-actin from long fibers to punctate structures.

Western Blot Analysis

Cell Line:	COS-7 cells expressing YFP-actin^[1]
Concentration:	0.3, 1, 3 and 10 μM
Incubation Time:	30 min
Result:	Concentration-dependently decreased the rate of actin depolymerization in COS-7 cells.

Clinical Trial

分子量

507.62

性状

Solid

Formula

C₃₀H₃₇NO₆

CAS 号

22144-77-0

中文名称

细胞松弛素D

结构分类

Others

来源

molds

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 25 mg/mL(49.25 mM;ultrasonic and warming and heat to 60℃)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	1.9700 mL	9.8499 mL	19.6998 mL
5 mM	0.3940 mL	1.9700 mL	3.9400 mL
10 mM	0.1970 mL	0.9850 mL	1.9700 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。