SID 3712249 (MiR-544 Inhibitor 1) 是一种miR-544生物合成抑制剂。SID 3712249 直接与前体 miRNA 结合。SID 3712249 阻断成熟 microRNA 的产生并减少 miR-544、HIF-1α 和 ATM 转录物。SID 3712249 可用于癌症研究。
| 生物活性 | SID 3712249 (MiR-544 Inhibitor 1) is amiR-544biogenesis inhibitor. SID 3712249 binds directly to the precursor miRNA. SID 3712249 blocks production of the maturemicroRNAand decreases miR-544, HIF-1α, andATMtranscripts. SID 3712249 can be used in the research of cancers, such as breastcancer[1]. |
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体外研究
(In Vitro) | SID 3712249 (compound 1, 20 nM, 48 h) disrupts miR-544-mediated inhibition of BMI1 based on significant increases in the RFP/BFP ratio[1].
SID 3712249 (20 nM, 48 h) inhibits precursor but not mature miR-544 binding to BMI1 or mTOR3’-UTR[1].
SID 3712249 (20 nM, 48 h) results in accumulation of pre-miR-544 and a decrease in miR-544 levels in MCF10A cells[1].
SID 3712249 (20 nM, 5 days) induces apoptosis in MDA-MB-231 and MCF- 7 cells and has no effect on cell survival in normoxic conditions[1].
Apoptosis Analysis[1] | Cell Line: | MDA-MB-231, MCF-7 cells | | Concentration: | 20 nM | | Incubation Time: | 5 days | | Result: | Induced apoptosis and has no effect on cell survival in normoxic conditions (flow cytometric analysis of Annexin V and PI stainning). |
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体内研究
(In Vivo) | SID 3712249 (compound 1, 100 μL of 40 μM, intraperitoneal injection) inhibited tumor growth in MDA-MB-231-GFP-luc tumor model[1].
SID 3712249 (20 nM, pre-treated GFP-labeled MDA-MB-231 cells, intraperitoneal injection) inhibited tumor growth in MDA-MB-231-GFP-luc tumor model[1].
| Animal Model: | MDA-MB-231-GFP-luc tumor model[1] | | Dosage: | 100 μL of 40 μM | | Administration: | Intraperitoneal injection | | Result: | Inhibited tumor growth (evidenced by live animal bioluminescent imaging) with no overt side effects.
Decreased levels of miR-544, ATM, and HIF-1α and increased levels of mTOR (resected tumor samples). |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: DMSO : 16.67 mg/mL(51.55 mM;Need ultrasonic) 配制储备液 | 1 mM | 3.0921 mL | 15.4607 mL | 30.9215 mL | | 5 mM | 0.6184 mL | 3.0921 mL | 6.1843 mL | | 10 mM | 0.3092 mL | 1.5461 mL | 3.0921 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 |
