Cycloleucine

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Cycloleucine

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

52-52-8

环亮氨酸

Cycloleucine 是一种 S-腺苷甲硫氨酸介导的甲基化的特异性抑制剂。Cycloleucine 是NMDA受体甘氨酸变构位点的拮抗剂，K_i值为 600 μM。Cycloleucine 在体外也是 ATP：L-蛋氨酸-S-腺苷基转移酶的竞争性抑制剂。Cycloleucine 具有抗焦虑和抑制细胞作用。

生物活性

Cycloleucine is a specific inhibitor of S-adenosyl-methionine mediated methylation. Cycloleucine is antagonist ofNMDAreceptor associated glycine receptor, with aK_iof 600 μM. Cycloleucine is also a competitive inhibitor of ATP: L-methionine-S-adenosyl transferase in vitro. Cycloleucine has anxiolytic and cytostatic effects^[1]^[2]^[3]^[4].

IC₅₀& Target

Ki: 600 μM (NMDA)^[1]^[2]

体外研究
(In Vitro)

Cycloleucine (4-40 mM; 3 h) blocks internal methylation of viral RNA in B77 transformed chick embryo fibroblasts^[5].
Cycloleucine (40 mM; 24 h) blocks the formation of both m⁶A and the penultimate G^min B77 38S RNA subunits by greater than 90%^[5].
Cytostatic (10 μg/mL) inhibits the viability human KB and mouse L1210s leukemia cell lines^[5].

体内研究
(In Vivo)

Cycloleucine (0.5-4 μg; intracerebroventrical injection) increases time spent in open arms, open arm entries, and extreme arrivals in rats^[3]. Cycloleucine reduces thymus and spleen weights inSemliki Forestvirus (SFV) strain A7(74) infected and control mice^[6].

Animal Model:	Male rats bilaterally cannulated into the nucleus accumbens septi (NAS)^[3]
Dosage:	1 μL of 0.5, 1.0, 2.0, 4 μg/μL
Administration:	Intracerebroventrical injection
Result:	Increased time spent in the open arms and extreme arrivals at all doses. Increased open arm entries at the dose of 4 μg.

分子量

129.16

性状

Solid

Formula

C₆H₁₁NO₂

CAS 号

52-52-8

中文名称

环亮氨酸；1氨基环戊烷甲酸

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

H₂O : 20 mg/mL(154.85 mM;Need ultrasonic)

DMSO :< 1 mg/mL (ultrasonic)(insoluble or slightly soluble)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	7.7423 mL	38.7117 mL	77.4233 mL
5 mM	1.5485 mL	7.7423 mL	15.4847 mL
10 mM	0.7742 mL	3.8712 mL	7.7423 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (774.23 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。