Lipopolysaccharides

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Lipopolysaccharides

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

脂多糖
LPS

Lipopolysaccharides (LPS) 脂多糖是从革兰氏阴性菌外膜的外叶中提取的内毒素，由一个抗原 O- 特异性链、一个核心寡糖和脂质 A 构成。Lipopolysaccharides 是一种激活免疫系统的致病相关分子模式 (PAMP)。Lipopolysaccharides 激活免疫细胞的TLR-4。本产品来源于大肠杆菌 O55:B5。Lipopolysaccharides 诱导细胞迁移体的分泌。

生物活性

Lipopolysaccharides (LPS) is an endotoxin derived from the outer leaflet of the outer membrane of Gram-negative bacteria. Lipopolysaccharides consists of an antigen O-specific chain, a core oligosaccharide and lipid A. Lipopolysaccharides is a pathogenic associated molecular pattern (PAMP) that activates the immune system. Lipopolysaccharides activatesTLR-4on immune cells^[1]^[2]^[3]. This product is derived fromEscherichia coliO55:B5. Lipopolysaccharides induces secretion of cell migrasome^[4].

IC₅₀& Target

TLR4

体外研究
(In Vitro)

Lipopolysaccharides (10–80 μg/mL) selectively decreases THir (tyrosine hydroxylase immunoreactive) cells and increases culture media levels of interleukin1β (IL-1β) and tumor necrosis factor-α (TNF-α) as well as nitrite (an index of nitric oxide (NO) production)^[5].

体内研究
(In Vivo)

Lipopolysaccharides (1.5 mg/kg; i.p.; once) induces sickness and hypothermia in mice, and induces a greater and more prolonged sickness response in adult male mice^[3].

Animal Model:	Female and male CD1 mice^[3]
Dosage:	1.5mg/kg
Administration:	Intraperitoneal injection, once
Result:	Induced sickness behavior in all mice, but adult mice displayed more sickness than pubertal mice and adult males remained sick for a longer period of time than adult females. Caused a decrease in body temperature for all mice, but this decrease was greatest in adult males. Increased pro- and anti-inflammatory cytokines at various levels in pubertal and adult male and female mice, resulted in age and sex differences in cytokine concentrations following immune challenge. Only adult males and females treated with LPS displayed significantly more IL-6 than their saline controls, and pubertal males and females and adult females displayed significantly more IL-10 than their saline controls. All the mice displayed significantly more IL-12 and TNF-α than their saline controls.

Clinical Trial

性状

Solid

中文名称

脂多糖

结构分类

Saccharides

来源

surface of Gram-negative bacteria

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

H₂O : 5 mg/mL(Need ultrasonic)

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： PBS
Solubility: 8.33 mg/mL (Infinity mM); Clear solution; Need ultrasonic and warming and heat to 60℃

*以上所有助溶剂都可在本网站选购。