Isoginkgetin

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Isoginkgetin

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

548-19-6

异银杏素

Isoginkgetin 是一种pre-mRNA splicing抑制剂。Isoginkgetin 也抑制Akt、NF-κB和MMP-9的活性。Isoginkgetin 抑制20S proteasome，诱导细胞凋亡（apoptosis），激活自噬（autophagy）。

生物活性

Isoginkgetin is apre-mRNA splicinginhibitor inhibitor. Isoginkgetin also inhibits activities of bothAkt,NF-κBandMMP-9. Isoginkgetin inhibits the activity of the20Sproteasome, inducesapoptosisand activatesautophagy^[1]^[2].

体外研究
(In Vitro)

Isoginkgetin (0-20 μM, 24 h) inhibits cell invasion in a dose-dependent manner^[1].
Isoginkgetin (10 μM, 0-24 h) induces the accumulation of ubiquitinated protein cargo into perinuclear aggregates, activates autophagy and induces lysosomal swelling, repositioning, and acidification^[2].
Isoginkgetin (10 μM, 0-6 h) induces ER stress, impairs the unfolded protein response, and results in accumulation of ERAD substrates^[2].
Isoginkgetin directly inhibits the chymotrypsin-, trypsin-, and caspase-like activities of the 20S proteasome and impairs NF-κB signaling^[2].
Isoginkgetin (30 μM, 0-24 h) induces apoptosis in MM cell lines^[2].

Cell Invasion Assay^[1]

Cell Line:	MDA-MB-231 and B16F10
Concentration:	0, 5, 10 and 20 μM
Incubation Time:	24 h
Result:	Inhibited cell invasion in a dose-dependent manner.

Western Blot Analysis^[2]

Cell Line:	HeLa cells and MM cell lines (MM1S, 8826, OPM2, H929, JJN3 and U266)
Concentration:	10 μM in HeLa cells, 30 μM in MM cell lines
Incubation Time:	0-24 h
Result:	Induced the expression of cleaved PARP-1 and Casp-3. Increased the protein levels of both ATF4 and ATF3. Decreased the level of LC3 I. Increased TFEB activation. Prevented the degradation of IκBα in response to stimulation with TNF-α.

Cell Viability Assay^[2]

Cell Line:	MM1S, 8826, OPM2, H929, JJN3 and U266 cells
Concentration:
Incubation Time:	72 h
Result:	Inhibited cell viability with IC₅₀values of 3.992, 3.716, 4.749, 2.564, 3.806 and 4.147 μM against MM1S, 8826, OPM2, H929, JJN3 and U266 cells, respectively.

体内研究
(In Vivo)

Isoginkgetin (4 mg/kg; i.p.; daily for 14 days) shows anti-inflammatory effects^[3].

Animal Model:	Adult male Kunming mice (age 8–10weeks, 30–50 g)^[3]
Dosage:	4 mg/kg
Administration:	Daily for 14 days by intraperitoneally (i.p.) prior to LPS (0.83mg/kg) administration.
Result:	Markedly suppressed the production of IL-1β, IL-6, tumor necrosis factor-alpha, cyclooxygenase-2, inducible nitric oxide, and reactive oxygen species.

分子量

566.51

性状

Solid

Formula

C₃₂H₂₂O₁₀

CAS 号

548-19-6

中文名称

异银杏素

结构分类

Flavonoids
Biflavones

Phenols
Polyphenols

来源

Plants
Ginkgoaceae
Ginkgo biloba

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 100 mg/mL(176.52 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	1.7652 mL	8.8260 mL	17.6519 mL
5 mM	0.3530 mL	1.7652 mL	3.5304 mL
10 mM	0.1765 mL	0.8826 mL	1.7652 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (3.67 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (3.67 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液
2.
请依序添加每种溶剂： 10% DMSO 90% (20%SBE-β-CDin saline)
Solubility: 2.08 mg/mL (3.67 mM); Suspended solution; Need ultrasonic
此方案可获得 2.08 mg/mL (3.67 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明

*以上所有助溶剂都可在本网站选购。