Coelonin 是一种具有抗炎活性的二氢菲。Coelonin 可抑制 LPS 诱导的PTEN磷酸化。Coelonin 通过负向调节PI3K/AKT通路抑制NF-κB激活和p27Kip1降解。Coelonin 可抑制IκBα的磷酸化和降解，增加 IκBα 蛋白的表达。
| 生物活性 | Coelonin is a dihydrophenanthrene with anti-inflammation activity. Coelonin inhibits LPS-inducedPTENphosphorylation. Coelonin inhibitsNF-κBactivation andp27Kip1degradation by regulating thePI3K/AKTpathway negatively. Coelonin can inhibitIκBαphosphorylation and degradation and increases the expression ofIκBαprotein[1][2]. |
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体外研究
(In Vitro) | Coelonin (2.5 μg/mL) 显著降低 NF-κB p65 和 p105/50 磷酸化水平[1]。
Coelonin (0-5 μg/mL, 1.5 h) 剂量依赖性地降低 LPS 诱导的 PTEN、AKT 和 IκBa 磷酸化的增加[1]。
Coelonin (10 和 20 μg/ml) 通过减少炎症因子(包括白细胞介素 6 (IL-6) 和肿瘤坏死因子-α (TNF-α)) 的产生来减轻颗粒物 2.5 (PM2.5) 诱导的炎症< sup>[2].
Coelonin 对 IL-1β、IL-6 和 TNF-α 表达的抑制与 PTEN 无关，而对 p27Kip1 降解的抑制导致细胞周期停滞在 G1 期，这依赖于 PTEN[1].
Western Blot Analysis[1] | Cell Line: | RAW264.7 cells | | Concentration: | 0, 1, 2.5, and 5 μg/mL | | Incubation Time: | 1.5 h | | Result: | Dose dependently reduced the increase of p65 accumulation in the nucleus induced by LPS. Dose dependently reversed LPS-induced iNOS and COX2 expression. LPS (200 ng/mL) significantly increased the phosphorylation of PTEN, AKT and inhibitor of NF-κB (IκBa), which was dose-dependently reduced by coelonin pre-treatment. |
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| 来源 | - Plants
- Orchidaceae
- Appendicula reflexaBlume
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
