PKI(5-24)

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PKI(5-24)

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CAS NO:

99534-03-9

PKI(5-24) 是一种强效、竞争性、合成的PKA(cAMP 依赖性蛋白激酶) 抑制剂，K_i为 2.3 nM。PKI(5-24) 对应于天然存在的热稳定蛋白激酶抑制剂中的 5-24 残基。

生物活性

PKI(5-24) is a potent, competitive, and synthetic peptide inhibitor ofPKA(cAMP-dependent protein kinase), with aK_iof 2.3 nM. PKI(5-24) corresponds to residues 5-24 in the naturally occurring heat-stable protein kinase inhibitor^[1]^[2].

体外研究
(In Vitro)

PKI(5-24) is a 20-residue peptide has been synthesized that corresponds to the active site of the skeletal muscle inhibitor protein^[1].
PKI(5-24) inhibits phosphotransferase activity of the mutant cGMP kinase with higher potency than that of wild type, with Ki values of 42 μΜ and 160 μΜ, respectively^[2].

分子量

2222.38

性状

Solid

Formula

C₉₄H₁₄₈N₃₂O₃₁

CAS 号

99534-03-9

Sequence

Thr-Thr-Tyr-Ala-Asp-Phe-Ile-Ala-Ser-Gly-Arg-Thr-Gly-Arg-Arg-Asn-Ala-Ile-His-Asp

Sequence Shortening

TTYADFIASGRTGRRNAIHD

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

H₂O : 25 mg/mL(11.25 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	0.4500 mL	2.2498 mL	4.4997 mL
5 mM	0.0900 mL	0.4500 mL	0.8999 mL
10 mM	0.0450 mL	0.2250 mL	0.4500 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。