Fluorescein diphosphate tetraammonium is afluorescent dye, it can be used as a fluorogenic substrate and generats fluorescein as a fluorescent product^[1][2][3].

Fluorescein diphosphate tetraammonium can be used as a fluorogenic substrate, and obtains luorescein as a fluorescent product^[1].
Fluorescein diphosphate tetraammonium (50 μM; room temperature) can be used as a substrate in vitro phosphatase assays^[2].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
ELISA with capillary immunosensor (single-step ELISA)^[3]:
1. Put various concentrations of antigen in Tris buffer 1 containing 1% BSA into capillary immunosensor.
2. Immobilize capture antibody and enzyme-linked antibody via capillary action, incubates for a preset time, and subsequently washing with 1 mL of 50 mM Tris buffer (pH 7.4) containing 0.14 M NaCl and 0.05% Tween 20 by using a syringe.
3. Fluorescence is detected by introducing an excess volume (ca. 100 μL) of 20 μM FDP in 50 mM Tris buffer (pH 9) with an incubation time of 60 min.

560.39

C₂₀H₂₆N₄O₁₁P₂

217305-49-2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.