Glucagon(1-29),bovine,human,porcine

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CAS NO:

16941-32-5

胰高血糖素
Porcine glucagon

Glucagon (1-29), bovine, human, porcine 是由胰腺 α 细胞产生的一种多肽激素。Glucagon 促进糖异生。Glucagon (1-29), bovine, human, porcine 可激活 HNF4α 并促进 HNF4α 磷酸化。

生物活性

Glucagon (1-29), bovine, human, porcine is a peptide hormone, produced by pancreatic α-cells. Glucagon stimulates gluconeogenesis^[1]. Glucagon (1-29), bovine, human, porcine activates HNF4α and increases HNF4α phosphorylation^[2]^[3].

体外研究
(In Vitro)

Upon binding to its receptor Gcgr, Glucagon activates cAMP-PKA signaling to stimulate hepatic glucose production (HGP) and cause hyperglycemia^[1].
Glucagon stimulates both hepatic kisspeptin1 production and gluconeogenesis^[1].
Glucagon (100 nM) represses CYP7A1 mRNA expression in human primary hepatocytes^[3].
Glucagon (100 nM) increases phosphorylayion of HNF4α^[3].

Western Blot Analysis^[3]

Cell Line:	Human primary hepatocytes (H1211, HH1215)
Concentration:	100 nM
Incubation Time:
Result:	Resulted in a marked increase in the amount of phosphorylated HNF4α.

体内研究
(In Vivo)

Low-dose (20 μg/kg) Glucagon increases glycemia and does not stimulate insulin secretion in ambient-fed mice. High-dose (1 mg/kg) Glucagon lowers glycemia compared with PBS control and stimulates insulin secretion in ambient-fed mice^[4].

Animal Model:	C57BL/6J mice (12- to 24-week-old)^[4]
Dosage:	20 μg/kg and 1 mg/kg
Administration:	Administered by i.p. injection; 45 minutes
Result:	Low-dose (20 μg/kg) increased glycemia and did not stimulate insulin secretion. High-dose (1 mg/kg) lowered glycemia and stimulated insulin secretion.

分子量

3482.75

性状

Solid

Formula

C₁₅₃H₂₂₅N₄₃O₄₉S

CAS 号

16941-32-5

Sequence

His-Ser-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr

Sequence Shortening

HSQGTFTSDYSKYLDSRRAQDFVQWLMNT

中文名称

胰高血糖素

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据

In Vitro:

H₂O : 6.67 mg/mL(1.92 mM;ultrasonic and adjust pH to 3 with HCl)

DMSO : 2.5 mg/mL(0.72 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	0.2871 mL	1.4356 mL	2.8713 mL
5 mM	---	---	---
10 mM	---	---	---

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline
Solubility: 2.5 mg/mL (0.72 mM); Suspended solution; Need ultrasonic
此方案可获得 2.5 mg/mL (0.72 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在本网站选购。