Hoechst 33342 trihydrochloride is a membrane permeant blue fluorescentDNAstain.

Dye reagent^[1]
DNA Stain^[1]

Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture. Hoechst 33342 can also be used to stain fixed cells by substituting Hoechst 33342 for DAPI^[1].

561.93

Solid

C₂₇H₃₁Cl₃N₆O

875756-97-1

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。

DMSO : ≥ 46 mg/mL(81.86 mM)

H₂O : ≥ 5.6 mg/mL(9.97 mM)

*"≥" means soluble, but saturation unknown.

请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： PBS

  Solubility: 3.33 mg/mL (5.93 mM); Clear solution; Need ultrasonic and warming and heat to 60℃

• 
  Description: Hoechst 33342 trihydrochloride can be used for nuclei staining with blue fluorescence.
• 
  Description: Hoechst 33342 trihydrochloride can be used for nuclei staining with blue fluorescence to detect apoptosis.
  Method: For cell staining.
  1. Stain cells with Hoechst 33342 trihydrochloride (10 min).
  2. Use a fluorescence microscope (Nikon) to image.
• 
  Description: Hoechst 33342 trihydrochloride can be used to label chromatin with blue fluorescence.
  Method: For cell staining.
  1. Chromatin is briefly counterstained with Hoechst 33342 trihydrochloride (5 μg/mL) before imaging.
  2. Use a confocal microscope (Leica SP8) for image.