RI-1 is aRAD51inhibitor, withIC₅₀s ranging from 5 to 30 μM. RI-1 binds covalently to the surface ofRAD51protein at cysteine 319. RI-1 inactivatesRAD51by directly binding to a protein surface that serves as an interface between protein subunits inRAD51filaments. RI-1 can disrupt homologous recombination in human cells^[1].

IC50: 5-30 μM (RAD51)^[1]

RI-1 (1-50 μM; 24 h) specifically inhibits homologous recombination (HR) in U2OS cells and stimulates single-strand annealing (SSA) in HEK293 cells^[1].
RI-1 (5-20 μM; 30 min) inhibits HsRAD51 in a concentration-dependent manner^[1].
RI-1 (20 μM; 8 h) disrupts the formation of RAD51 foci after DNA damage in immortalized human fibroblasts^[1].
RI-1 (15-25 μM; 24 h) sensitizes human cancer cells (HeLa, MCF-7 and U2OS) to cross-linking chemotherapy^[1].

RI-1 (50 mg/kg; i.p. every 3 d for 30 d) significantly reduces triple negative breast cancer (TNBC) tumor growth in mice^[2].

361.61

Solid

C₁₄H₁₁Cl₃N₂O₃

415713-60-9

Room temperature in continental US; may vary elsewhere.

DMSO : 33.33 mg/mL(92.17 mM;Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMSO    90%corn oil

  Solubility: ≥ 2.5 mg/mL (6.91 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (6.91 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。