HAEGT

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HAEGT

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

852155-81-8

HAEGT 是胰高血糖素样肽-1 (GLP-1) 的多肽 N-端的 1-5 残基片段，序列为 His-Ala-Glu-Gly-Thr。HAEGT 可作为竞争性底物检测人二肽基肽-IV (DPP-IV) 的底物结合位点，其 N 端His-Ala会被DPP-IV催化裂解。HAEGT 可用于糖尿病、肥胖等疾病的研究。

生物活性

HAEGT is the first N-terminal 1-5 residues ofglucagon like peptide-1 (GLP-1)peptide, and the sequence isHis-Ala-Glu-Gly-Thr. HAEGT acts as a competitive substrate for probing prime substrate binding sites of humandipeptidyl peptidase-IV (DPP-IV) 1, in which the N-terminalHis-Alais catalyzed cleavage by DPP-IV. HAEGT can be used in the research of diabetes, obesity^[1].

IC₅₀& Target^[1]

DPP-4

体外研究
(In Vitro)

HAEGT (0-500 μM) is a competitive substrate for probing prime substrate binding sites of human dipeptidyl peptidase-IV (DPP-IV)^[1].
HAEGT can be catalyzed cleavage by DPP-IV with a k_mvalue of 38 μM, K_catvalue of 3.1 S^-1^[1].

分子量

513.50

性状

Solid

Formula

C₂₀H₃₁N₇O₉

CAS 号

852155-81-8

Sequence

His-Ala-Glu-Gly-Thr

Sequence Shortening

HAEGT

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O

Peptide Solubility and Storage Guidelines:

1. Calculate the length of the peptide.

2. Calculate the overall charge of the entire peptide according to the following table:

	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3. Recommended solution:

Overall charge of peptide	Details
Negative (<0)	1. Try to dissolve the peptide in water first. 2. If water fails, add NH₄OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)	1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.