L-NIL

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L-NIL

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

53774-63-3

L-NIL 是诱导型一氧化氮合成酶(iNOS)的抑制剂，其对 miNOS 的IC₅₀值为3.3 μM。

生物活性

L-NIL is aninducibleNO synthaseinhibitor, with anIC₅₀of 3.3 μM for miNOS^[1]^[2]^[3].

IC₅₀& Target

IC50: 3.3 μM (mouse inducible NO synthase), 92 μM (rat brain constitutive NO synthase)^[1]

体外研究
(In Vitro)

L-NIL produces a concentration-dependent inhibition of both the mouse inducible NOS (miNOS) and the rat brain constitutive NOS (rcNOS) and is considerably more potent for miNOS. The IC₅₀values for L-NIL with miNOS and rcNOS are 3.3 and 92 pM, respectively, indicating that L-NIL is 28-fold more selective for miNOS. In addition, L-NIL has approximately 6-fold greater potency for miNOS than either L-NMA or L-NNA^[3].

体内研究
(In Vivo)

L-NIL (10 and 30 mg/kg, IP) prevents the inflammation, oxidative stress and autophagy induced by renal IR in mice^[1].

Animal Model:	Adult male Balb/c (20-25 g)^[1].
Dosage:	10 and 30 mg/kg.
Administration:	Intraperitoneally at the end of CLP and at 6 h after sepsis induction.
Result:	Led to a negligible increase in plasma NGAL compared to sham mice. Led to a significant decrease in both TLR4 and IL1β?protein contents and clusterin transcript. Showed an increase in NFAT5 mRNA levels, as compared with mice treated with vehicle. Promoted a decrease in AR protein expression, as compared with animals treated with vehicle.

分子量

187.24

性状

Solid

Formula

C₈H₁₇N₃O₂

CAS 号

53774-63-3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

H₂O : 50 mg/mL(267.04 mM;Need ultrasonic)

DMSO :< 1 mg/mL(insoluble or slightly soluble)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	5.3407 mL	26.7037 mL	53.4074 mL
5 mM	1.0681 mL	5.3407 mL	10.6815 mL
10 mM	0.5341 mL	2.6704 mL	5.3407 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (534.07 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。