cGAMP disodium

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cGAMP disodium

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CAS NO:

2407516-83-8

Cyclic GMP-AMP disodium
3',3'-cGAMP disodium

cGAMP (Cyclic GMP-AMPP) disodium 作为内源性第二信使发挥作用，并触发干扰素的产生以响应胞浆DNA。cGAMP disodium 激活干扰素基因刺激因子(STING)，激活导致产生 I 型干扰素和其他免疫介质的信号级联。

生物活性

cGAMP (Cyclic GMP-AMPP) disodium functions as an endogenous second messenger in metazoans and triggers interferon production in response to cytosolic DNA. cGAMP diammonium activates stimulator of interferon genes(STING), which activates a signaling cascade leading to the production of type I interferons and other immune mediators^[1]^[2]^[3]^[4].

体外研究
(In Vitro)

cGAMP disodium promotes the antigen-specific proliferation capacity of spleen cells in mice^[2].
cGAMP disodium directly activates murine and human dendritic cells in vitro^[2].
On stimulation with cGAMP disodium, fibroblasts from the patients showed increased transcription of IFNB1 but not of the genes encoding interleukin-1 (IL1), interleukin-6 (IL6), or tumor necrosis factor (TNF)^[3].
cGAMP disodium activates the endoplasmic reticulum (ER)-resident receptor STING, thereby inducing an antiviral state and the secretion of type I IFNs^[4].

体内研究
(In Vivo)

cGAMP (5 μg; nostril mucosal adjuvant) disodium promotes the antigen-specific cytokine production by spleen cells of immunized mice^[2].

Animal Model:	Female C57BL/6 (H-2b) mice 6-8 weeks old^[2]
Dosage:	5 μg
Administration:	Nostril mucosal adjuvant
Result:	Higher titers of ovalbumin (OVA)-specific IgA and total IgG as well as IgG1 and IgG2c in the sera of mice immunized with cGAMP-adjuvanted OVA as compared to sera from OVA-immunized mice.

分子量

718.37

性状

Solid

Formula

C₂₀H₂₂N₁₀Na₂O₁₃P₂

CAS 号

2407516-83-8

结构分类

Ketones, Aldehydes, Acids

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

H₂O : 180 mg/mL(250.57 mM;Need ultrasonic and warming)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	1.3920 mL	6.9602 mL	13.9204 mL
5 mM	0.2784 mL	1.3920 mL	2.7841 mL
10 mM	0.1392 mL	0.6960 mL	1.3920 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (139.20 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。