3-Bromopyruvic acid

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3-Bromopyruvic acid

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

1113-59-3

3-溴丙酮酸
Bromopyruvic acid
Hexokinase II Inhibitor II, 3-BP

3-Bromopyruvic acid 是己糖激酶 II (hexokinase II,HXK II) 抑制剂，是一种有效的针对肝癌细胞的抗肿瘤剂。

生物活性

3-Bromopyruvate (Bromopyruvic acid) is an analogue of pyruvate and a potenthexokinase(HK)-IIinhibitor with high tumor selectivity. 3-Bromopyruvate inhibits cell growth and inducesapoptosisthrough interfering with glycolysis. 3-Bromopyruvate inducesautophagyby stimulating ROS formation in breastcancercells. Antimicrobial activities^[1]^[2]^[3].

体外研究
(In Vitro)

3-Bromopyruvate enhances TRAIL-induced apoptosis in breast cancer cells^[2].
3-Bromopyruvate (Bromopyruvic acid), a hexokinase II inhibitor, can induce apoptosis in hepatocellular carcinoma cells by inducing endoplasmic reticulum (ER) stress^[2].
3-Bromopyruvate inhibits ATP generation and upregulates the expression of DR5. 3-Bromopyruvate upregulates CHOP, GRP78 and the phosphorylation of AMPK and augments TRAIL-induced Bax and caspase-3 levels^[2].

Cell Viability Assay^[2]

Cell Line:	MCF-7 and MDA-MB-231 cells
Concentration:	40, 80, 160 or 320 μM
Incubation Time:	24 hours
Result:	3-Bromopyruvate (80 and 160 μmol/l) and TRAIL (400 ng/ml) significantly inhibited cell viability.

体内研究
(In Vivo)

3-Bromopyruvate (8 mg/kg; i.p.; every 4 days for 28 days) shows a synergistic antitumor effect in MCF-7 cell xenografts in nude mice^[2].

Animal Model:	Female nude mice (BALB/c; 4-5-weeks old and 18-20 g)^[2]
Dosage:	8 mg/kg
Administration:	I.p.; every 4 days for 28 days
Result:	Showed antitumor efficacy in tumor xenografts.

分子量

166.96

性状

Solid

Formula

C₃H₃BrO₃

CAS 号

1113-59-3

中文名称

3-溴丙酮酸；溴丙酮酸；3-溴庚酸；3-溴代丙酮酸

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

H₂O : 250 mg/mL(1497.36 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	5.9895 mL	29.9473 mL	59.8946 mL
5 mM	1.1979 mL	5.9895 mL	11.9789 mL
10 mM	0.5989 mL	2.9947 mL	5.9895 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (598.95 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。