| In Vitro | In vitro activity: Previous research showed hexylresorcinol could inhibit both mono- and di-phenolase activity of mushroom tyrosinase. Moreover, hexylresorcinol at 2 μM lengthened the lag period from 98 s to 26. Hexylresorcinol could also display reversible inhibition of the enzyme. In addition, the kinetic analyses showed that hexylresorcinol was a competitive inhibitor with the apparent inhibition constant binding with free enzyme to be 0.443 μM for diphenolase
Kinase Assay:
Cell Assay: |
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| In Vivo | Iin vivo study showed that hexylresorcinol could induce chromosome aberrations in mouse eukaryotic cells at doses of 0.5, 0.05, and 0.005 mg/g and the metabolic transformation of hexylresorcinol decreased its genotoxic effect in mice. Moreover, the mutagenic effect lasted for 3 days only at the highest dose of hexylresorcinol (0.5 mg/g). Thus, hexylresorcinol doses less than 0.5 mg/g were metabolized within two days to the extent of the cytotoxic effect. In addition, hexylresorcinol was transformed at a rate of 0.0025–0.025 mg/day after a single administration to mice |
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