Salvigenin 是一种天然的多酚类化合物，具有神经保护作用。Salvigenin 具有抗肿瘤、细胞毒和免疫调节作用。Salvigenin 抑制H2O2诱导的细胞凋亡（apoptosis。
| 生物活性 | Salvigenin is a natural polyphenolic compound, with neuroprotective effect. Salvigenin has antitumor cytotoxic and immunomodulatory properties. Salvigenin inhibits H2O2-induced cellapoptosis[1][2]. |
体外研究
(In Vitro) | Salvigenin (0-100 μM, 3 h) protects SH-SY5Y cells against H2O2-induced cell death[1].
Salvigenin (0-100 μM, 2 h) significantly reduces ROS generation, increases the glutathione level, declines the extent of cell apoptosis and increases autophagy in H2O2-exposed SH-SY5Y cells[1].
Cell Viability Assay[1] | Cell Line: | SY5Y cells exposed to H2O2(300 μM) | | Concentration: | 10, 25, 50, and 100 μM | | Incubation Time: | 3 h | | Result: | Protected SH-SY5Y cells (best in 25 and 50μM) against H2O2-induced cell death. |
Apoptosis Analysis[1] | Cell Line: | SY5Y cells exposed to H2O2(300 μM) | | Concentration: | 10, 25, 50, and 100 μM | | Incubation Time: | 2 h | | Result: | Significantly declined the extent of cell apoptosis induced by H2O2. |
Western Blot Analysis[1] | Cell Line: | SY5Y cells exposed to H2O2(300 μM) | | Concentration: | 10, 25, 50, and 100 μM | | Incubation Time: | 2 h | | Result: | Reduced cleaved caspase-3 and Bax/Bcl-2 ratio. Reduced caspase-12 and calpain levels. Reduced the level of Atg7, Atg12, and LC3-II/ LC3-I. |
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体内研究
(In Vivo) | Salvigenin (0-9.68 μg/mouse/day; i.p.; 4 or 12 days) shows antitumor and immunomodulatory effects on tumor bearing mice[2].
| Animal Model: | MCF-7 cell bearing inbred female Balb/c mice, aged from six to eight weeks[2] | | Dosage: | 3.65, 5.85 and 9.68 μg/mouse/day for four days or 12 days | | Administration: | Intraperitoneal injection | | Result: | Showed a significant increase in DTH response in a dose-dependent manner. Significantly decreased the rate of tumor growth, increased lymphocyte proliferation index, increased the level of IFN-γ and decreased IL-4 production. Exhibited a significant decrease of the splenic CD4+CD25+Foxp3+T lymphocytes. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
| 溶解性数据 | In Vitro: DMSO : 20 mg/mL(60.92 mM;ultrasonic and warming and heat to 60℃) 配制储备液 | 1 mM | 3.0458 mL | 15.2290 mL | 30.4581 mL | | 5 mM | 0.6092 mL | 3.0458 mL | 6.0916 mL | | 10 mM | 0.3046 mL | 1.5229 mL | 3.0458 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: 2 mg/mL (6.09 mM); Suspended solution; Need ultrasonic
此方案可获得 2 mg/mL (6.09 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。 以 1 mL 工作液为例，取 100 μL 20.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 *以上所有助溶剂都可在本网站选购。
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