首页 蛋白库 基因库 产品 会员服务
位置：首页 > 产品库 > 7α,25-dihydroxy Cholesterol
立即咨询
咨询类型：
     
*姓名：
*电话：
*单位：
Email：
*留言内容：
请详细说明您的需求。
*验证码：
 
7α,25-dihydroxy Cholesterol
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
7α,25-dihydroxy Cholesterol图片
CAS NO:64907-22-8
包装与价格：
包装价格(元)
1mg询价
5mg询价
10mg询价

7α, 25-dihydroxycholesterol (7α,25-OHC) 是孤儿 GPCR 受体 EBI2 (GPR183) 的有效选择性激动剂和内源性配体。
Cas No.64907-22-8
别名7Α,25-二羟基胆固醇,7α,25-DHC
化学名(3β,7α)-cholest-5-ene-3,7,25-triol
Canonical SMILESO[C@@H]1C=C2C[C@@H](O)CC[C@]2(C)[C@]3([H])[C@]1([H])[C@@](CC[C@]4([H])[C@H](C)CCCC(C)(C)O)([H])[C@]4(C)CC3
分子式C27H46O3
分子量418.7
溶解度≤2mg/ml in ethanol;0.1mg/ml in DMSO;20mg/ml in dimethyl formamide
储存条件Store at -20℃
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述

7α,25-dihydroxy Cholesterol (7α,25-DHC, 7α,25-OHC) is a potent and selective GPR183 agonist [1].

Epstein-Barr virus (EBV)-induced gene 2 (EBI2, also known as GPR183) is an orphan G protein-coupled receptor that is highly expressed in spleen and up-regulated upon Epstein–Barr-virus infection. GPR183 is required for humoral immune responses and polymorphisms in the receptor is associated with inflammatory autoimmune diseases [1][2].

7α,25-dihydroxy Cholesterol is a potent and selective GPR183 (EBI2) agonist and most potent EBI2 ligand with EC50 and Kd values of 140 pM and 450 pM, respectively. In a competition binding study, 7α,25-OHC competed for binding with IC50 value of 242 pM. In EBI2-expressing cells, 7α,25-OHC dose-dependently inhibited forskolin-induced cAMP with IC50 value of 2 nM. 7α,25-OHC was probably the endogenous ligand for EBI2. In EBI2-expressing mouse B and T cells, 7α,25-dihydroxy Cholesterol stimulated cell migration with IC50 value of around 500 pM, but had no effect on EBI2-deficient cells [1].

In EBI2-deficient mice, unseparated splenocytes, B cells and T cells were completely unresponsive to 7α,25-OHC stimulation [1]. Mice deficient in Ch25h, a key enzyme for the generation of 7α, 25-OHC, failed to position activated B cells within the spleen to the outer follicle [2].

References:
[1].  Liu C, Yang XV, Wu J, et al. Oxysterols direct B-cell migration through EBI2. Nature. 2011 Jul 27;475(7357):519-23.
[2].  Hannedouche S, Zhang J, Yi T, et al. Oxysterols direct immune cell migration via EBI2. Nature. 2011 Jul 27;475(7357):524-7.

 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024