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Suc-Leu-Leu-Val-Tyr-AMC
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Suc-Leu-Leu-Val-Tyr-AMC图片
CAS NO:94367-21-2

Suc-Leu-Leu-Val-Tyr-AMC 是一种荧光底物。
生物活性

Suc-Leu-Leu-Val-Tyr-AMC is a fluorogenic substrate.

体外研究
(In Vitro)

Suc-Leu-Leu-Val-Tyr-AMC (Suc-LLVY) is a membrane-permeable calpain-specific fluorogenic substrate, pteolytic hydrolysis of the peptidyl-7-amino bond liberates the highly fluorescent 7-amino-4-methylcoumarin (AMC) moiety[1]. The effect of TGF-β on hydrolysis of these substrates (e.g Suc-Leu-Leu-Val-Tyr-AMC) are assessed. Biliary epithelial H69 cells are incubated with 10, 1, 0.1, or 0 ng/mL TGF-β for 24 h. Substrate hydrolysis is then fluorometrically assessed in cytosolic extracts. Basal activity is 1.12, 8.33, and 14.52 nmol AMC/mg protein/min for suc-LLVY-AMC, z-LLE-AMC, and z-LLL-AMC hydrolysis, respectively[2].

分子量

763.88

性状

Solid

Formula

C40H53N5O10

CAS 号

94367-21-2

Sequence

Suc-Leu-Leu-Val-Tyr

Sequence Shortening

Suc-LLVY

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light, under nitrogen

Powder-80°C2 years
-20°C1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

溶解性数据
In Vitro: 

DMSO : ≥ 20 mg/mL(26.18 mM)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.3091 mL6.5455 mL13.0911 mL
5 mM0.2618 mL1.3091 mL2.6182 mL
10 mM0.1309 mL0.6546 mL1.3091 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

 
 
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