CAS NO: | 94367-21-2 |
生物活性 | Suc-Leu-Leu-Val-Tyr-AMC is a fluorogenic substrate. | ||||||||||||||||
体外研究 (In Vitro) | Suc-Leu-Leu-Val-Tyr-AMC (Suc-LLVY) is a membrane-permeable calpain-specific fluorogenic substrate, pteolytic hydrolysis of the peptidyl-7-amino bond liberates the highly fluorescent 7-amino-4-methylcoumarin (AMC) moiety[1]. The effect of TGF-β on hydrolysis of these substrates (e.g Suc-Leu-Leu-Val-Tyr-AMC) are assessed. Biliary epithelial H69 cells are incubated with 10, 1, 0.1, or 0 ng/mL TGF-β for 24 h. Substrate hydrolysis is then fluorometrically assessed in cytosolic extracts. Basal activity is 1.12, 8.33, and 14.52 nmol AMC/mg protein/min for suc-LLVY-AMC, z-LLE-AMC, and z-LLL-AMC hydrolysis, respectively[2]. | ||||||||||||||||
分子量 | 763.88 | ||||||||||||||||
性状 | Solid | ||||||||||||||||
Formula | C40H53N5O10 | ||||||||||||||||
CAS 号 | 94367-21-2 | ||||||||||||||||
Sequence | Suc-Leu-Leu-Val-Tyr | ||||||||||||||||
Sequence Shortening | Suc-LLVY | ||||||||||||||||
运输条件 | Room temperature in continental US; may vary elsewhere. | ||||||||||||||||
储存方式 | Sealed storage, away from moisture and light, under nitrogen
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen) | ||||||||||||||||
溶解性数据 | In Vitro: DMSO : ≥ 20 mg/mL(26.18 mM) *"≥" means soluble, but saturation unknown. 配制储备液
* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
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