Sulforhodamine B sodium salt

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CAS NO:

3520-42-1

Acid Red 52
Kiton Red 620

Sulforhodamine B sodium salt 是一种荧光染料，可用于定量测定培养细胞的细胞蛋白量。

生物活性

Sulforhodamine B sodium salt is afluorescent dyewith uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells.

体外研究
(In Vitro)

Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies. This method relies on the property of SRB, which binds stoichiometrically to proteins under mild acidic conditions and then can be extracted using basic conditions; thus, the amount of bound dye can be used as a proxy for cell mass, which can then be extrapolated to measure cell proliferation. The protocol can be divided into four main steps: preparation of treatment, incubation of cells with treatment of choice, cell fixation and SRB staining, and absorbance measurement. This assay is limited to manual or semiautomatic screening, and can be used in an efficient and sensitive manner to test chemotherapeutic drugs or small molecules in adherent cells. It also has applications in evaluating the effects of gene expression modulation (knockdown, gene expression upregulation), as well as to study the effects of miRNA replacement on cell proliferation^[1].

分子量

580.65

性状

Solid

Formula

C₂₇H₂₉N₂NaO₇S₂

CAS 号

3520-42-1

中文名称

磺酰罗丹明 B；柴林红 B；奇通红 S；酸性桃红 B；酸性红 52；二甲苯红 B；酸性玫瑰红 B；磺化罗丹明 B；硫罗丹明 B；食品红 106

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据

In Vitro:

DMSO : 50 mg/mL(86.11 mM;Need ultrasonic)

H₂O : 20 mg/mL(34.44 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	1.7222 mL	8.6110 mL	17.2221 mL
5 mM	0.3444 mL	1.7222 mL	3.4444 mL
10 mM	0.1722 mL	0.8611 mL	1.7222 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： PBS
Solubility: 50 mg/mL (86.11 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。