Doxycycline calcium

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Doxycycline calcium

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

94088-85-4

Doxycycline calcium 是一种具有口服活性的四环素抗生素，是广谱的金属蛋白酶 (MMP) 抑制剂，具有抗菌活性和抗癌细胞增殖活性。

生物活性

Doxycycline calcium, anantibiotic, is an orally active and broad-spectrum metalloproteinase (MMP) inhibitor^[1]. Doxycycline calcium shows antibacterial activity and anti-cancer cell proliferation activity^[1]^[2]^[3]^[4]^[5].

IC₅₀& Target

Tetracycline

体外研究
(In Vitro)

Doxycycline calcium (0.01-10 μg/mL, 4 d) affects growth of glioma cells only under high concentrations^[2].
Doxycycline calcium (0.01-10 μg/mL, 24 h) decreases MT-CO1 protein content with concentrations of 1 μg/mL and higher in SVG cells^[2].
Doxycycline calcium (100 ng/mL, 1 μg/mL; 24 h) reduces proliferation of human cell lines^[4].
Doxycycline calcium (0-250 μM, 72 h) inhibits cell viability of breast cancer cells^[5].

Cell Viability Assay^[2]

Cell Line:	LNT-229, G55, and U343 glioma cells
Concentration:	0.01, 0.1, 1 or 10 μg/mL
Incubation Time:	4 days
Result:	Affected growth of glioma cells only under high concentration (10 μg/mL).

Cell Viability Assay^[2]

Cell Line:	SVG cells
Concentration:	0.01, 0.1, 1 or 10 μg/mL
Incubation Time:	24 hours
Result:	Decreaseed MT-CO1 protein content with concentrations of 1 μg/mL and higher.

Cell Proliferation Assay^[4]

Cell Line:	MCF 12A, 293T cells
Concentration:	100 ng/mL, 1 μg/mL
Incubation Time:	96 hours
Result:	Caused reduced proliferation of MCF 12A and 293T cells at 1 μg/mL.

Cell Viability Assay^[5]

Cell Line:	MCF-7, MDA-MB-468 cells
Concentration:	0-250 μM
Incubation Time:	72 hours
Result:	Inhibited breast cancer cells in a dose-dependent manner with IC₅₀values for MCF-7 and MDA-MB-468 of 11.39 μM and 7.13 μM respectively.

体内研究
(In Vivo)

Doxycycline calcium (oral gavage; 200 or 800 mg/kg; once daily; 3 months) reduces MMP-9 activity in untreated HT mice in a dose-dependent manner^[3].

Animal Model:	6-month-old female Heterozygous Col3a1-deficient (HT) mice^[3]
Dosage:	200 or 800 mg/kg
Administration:	Oral gavage; 200 or 800 mg/kg; once daily; 3 months
Result:	Reduced MMP-9 activity in a dose-dependent manner.

Clinical Trial

分子量

524.59

Formula

C₂₂H₂₄Ca₂N₂O₈

CAS 号

94088-85-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.