CAS NO: | 1811539-88-4 |
包装: | 2mg |
市场价: | 5233元 |
Cas No. | 1811539-88-4 |
别名 | JF585, SE; JF585, NHS |
Canonical SMILES | O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CC(F)(F)C4)C=C3C5(C)C)=C(C=C/6)C5=CC6=[N+]7CC(F)(F)C\7)=C2 |
分子式 | C34H27F4N3O6 |
分子量 | 649.6 |
溶解度 | Soluble in DMSO |
储存条件 | Store at -20°C |
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. |
Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
产品描述 | Orange fluorogenic fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 585 nm; emission maximum = 609 nm; Quantum yield = 0.78; Max extinction coefficient = 156,000 M-1cm-1 (measured in Ethanol or TFE plus 0.1% TFA); Correction factor = 0.0990. Exhibits low non-specific background staining. To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA. We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals. HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc. Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID:28869757 |
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