Betulonic acid belongs to the pentacyclic triterpenic derivative class, has antitumor activities.In vitro: BEA-NP is found over three-times more permeable than that solubilized by DMSO in Caco-2 cell monocultures.[1]In vivo: The tumor growth in the S180 berry mice orally doses with BEA-NP at 75 mg/kg is inhibited by 50%. Rubusoside is effective in solubilizing BEA, maintaining its cytotoxicity, enhancing its permeability and reducing tumor growth when orally administered.[1] antitumor activities against MGC-803, PC3, Bcap-37, A375, and MCF-7 human cancer cell lines In vivo: The animals are treated with betulonic acid amide (50 mg/kg in Tween aqueous solution) and heptral (6 mg/kg) as hepatoprotective compounds. It is found that betulonic acid amide stimulats the regenerative response in hepatocytes under conditions of combined toxic exposure and promots recovery of their qualitative and quantitative characteristics. [2]

References:
[1]. Zhang J et al. Employing rubusoside to improve the solubility and permeability of antitumor compound betulonic acid. Nanomedicine (Lond). 2016 Oct 19.
[2]. Semenov DE et al. Hepatoprotective properties of betulonic acid amide and heptral in toxic liver injury induced by carbon tetrachloride in combination with ethanol.Bull Exp Biol Med. 2015 Jan;158(3):336-41.