Cell experiment:

MT-2 cells are pre-incubated to the bamboo leaf extract solution (up to 10%), Sodium copper chlorophyllin (up to 5 mM) or test reagent-free culture medium for 10 min to assess the inhibitory activity at adsorption. The pre-incubated cells are washed extensively to be able to eliminate antiviral activity of test reagents remaining in the culture medium, and then they are exposed to HIV-1LAI (1.3×10-4 TCID50 per cell). In addition to these pre-incubated cells, control wells are prepared to evaluate growth of the cells in a virus-free condition. Then, virus-exposed and mock-exposed cells are cultured in the absence of test reagents for three days. The total viable cells are counted on day 3[1].

Sodium copper chlorophyllin exerts antiviral activities against Influenza virus and HIV with IC50s of 50 to 100 μM for both of them.

Sodium copper chlorophyllin exhibits influenza virus adsorption at 200 µM (P<0.05). The 50% inhibitory concentration (IC50) and the 50% cytotoxic concentration (CC50) of Sodium copper chlorophyllin lay from 50 to 100 µM and 200 to 400 µM, respectively. Sodium copper chlorophyllin also inhibits HIV adsorption at 2.5 mM (P<0.05). The IC50 and CC50 of Sodium copper chlorophyllin lay from 50 to 100 µM and 200 to 400 µM, respectively[1].

[1]. Akiko Ito, et al. In Vitro Inhibition of Cytopathic Effect of Influenza Virus and Human Immunodeficiency Virus by Bamboo Leaf Extract Solution and Sodium Copper Chlorophyllin. Yonago Acta Med. 2016 Mar; 59(1): 61-65.