Dehydrobruceine B 是一种苦木素，可从鸦胆子 (Brucea javanica) 中分离得到。Dehydrobruceine B 与 Cisplatin (HY-17394) 通过线粒体途径，协同诱导细胞凋亡 (apoptosis)。Dehydrobruceine B 还增加凋亡诱导因子 (AIF) 和Bax表达，抑制Keap1-Nrf2。
| 生物活性 | Dehydrobruceine B, a quassinoid, can be isolated fromBrucea javanica. Dehydrobruceine B shows a synergistic effect with Cisplatin (HY-17394) to induceapoptosisvia mitochondrial method. Dehydrobruceine B increasesapoptosis-inducing factor (AIF)andBaxexpression and suppressesKeap1-Nrf2[1]. |
| IC50& Target[1] | |
体外研究
(In Vitro) | Dehydrobruceine B (1 μM; 48 h) 和 Cisplatin (3-18 μM; 48 h) 在 A549 细胞中具有协同作用，致使细胞毒性和凋亡效应[1]。
Dehydrobruceine B (1 μM; 24 h) 在 Cisplatin (3 μM, 6 μM; 24 h) 处理的A549细胞中，诱导胞内 ROS 产生[1]。
Dehydrobruceine B (3 μM, 6 μM; 24 h) 在 Cisplatin (3 μM, 6 μM; 24 h) 处理的A549细胞中，促进线粒体膜电位 (MMP) 的去极化和细胞色素 c 的易位[1]。
Dehydrobruceine B (1 μM; 24 h) 增强 A549 细胞抗凋亡和促凋亡蛋白水平的变化[1]。
Western Blot Analysis[1] | Cell Line: | A549 cells | | Concentration: | 1 μM; with or without Cisplatin | | Incubation Time: | 48 hours | | Result: | Upregulated the protein level of Bax, while downregulated the levels of Bcl-2 and Bcl-xL.
Enhanced caspase activation and PARP cleavage. |
Apoptosis Analysis[1] | Cell Line: | A549 cells | | Concentration: | 1 μM; with or without Cisplatin | | Incubation Time: | 48 hours | | Result: | Inhibited cell viability with 9 μM and 18 μM Cisplatin, respectively.
Induced cell apoptosis with 3 μM and 6 μM Cisplatin, respectively. |
Immunofluorescence[1] | Cell Line: | A549 cells | | Concentration: | 1 μM; with or without 3 μM and 6 μM Cisplatin, respectively | | Incubation Time: | 24 hours | | Result: | Resulted apoptosis-inducing factor (AIF) translocated from cytosol into nucleus dramatically in the co-treatment condition. |
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| 来源 | B. antidysenterica and B. guineensis |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
