CYD-4-61 是一种用于乳腺癌研究的新型Bax激活剂。CYD-4-61 抑制三阴性乳腺癌 MDA-MB-231 和 ER 阳性乳腺癌 MCF-7 细胞系增殖。CYD-4-61 激活Bax蛋白诱导细胞色素 c 释放，调节凋亡生物标志物，导致癌细胞凋亡 (apoptosis)。
| 生物活性 | CYD-4-61 is a novelBaxactivator used for breastcancerresearch. CYD-4-61 inhibits triple-negative breastcancerMDA-MB-231 and ER-positive breastcancerMCF-7 cell lines proliferation. CYD-4-61 activatesBaxprotein to induce cytochrome c release and regulate apoptotic biomarkers, leading tocancercellapoptosis[1]. |
| IC50& Target[1] | |
体外研究
(In Vitro) | CYD-4-61 (compound 49) (0-10 μM; 72 h) exhibits significantly improved antiproliferative activity with IC50s of 0.07 μM against triple-negative breast cancer MDA-MB-231 and 0.06 μM against ER-positive breast cancer MCF-7 cell lines, respectively[1].
CYD-4-61 (1 μM; 72 h) induces apoptosis among MCF-7 and MDA-MB-231 cells[1].
CYD-4-61 (5 μM and 10 μM; ) increases the level of cells with apoptotic bodies in MDA-MB-231 and MCF-7 cells[1].
CYD-4-61 (5 μM; 0-48 h) decreases the level of p-Bax, but increases the levle of total Bax protein, cytochrome c, and several protein markers related to apoptosis[1].
Cell Viability Assay[1] | Cell Line: | MCF-10A, T47D, MCF-7, MDA-MB-231, and MDA-MB-468 cells | | Concentration: | 0.1, 1, and 10 μM | | Incubation Time: | 72 hours | | Result: | Inhibited triple-negative breast cancer MDA-MB-231 and ER-positive breast cancer MCF-7 cell lines proliferation and viability. |
Western Blot Analysis[1] | Cell Line: | MCF-7 and MDA-MB-231 cells | | Concentration: | 5 μM | | Incubation Time: | 0, 6, 12, 24, and 48 hours | | Result: | Dose-dependently led to the upregulation of cleaved PARP-1, cleaved caspase 3, and downregulation of cyclin D1. |
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体内研究
(In Vivo) | CYD-4-61 (2.5 mg/kg; i.p.; once daily for 7 d) inhibits triple-negative breast tumor growth in xenograft model in mice[1].
| Animal Model: | Triple-negative breast cancer MDA-MB-231 xenograft model in mice[1] | | Dosage: | 2.5 mg/kg | | Administration: | Intraperitoneal injection; once daily for 7 consecutive days | | Result: | Significantly suppressed the growth of MDA-MB-231 tumors with an inhibition rate of 55%. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
