SP-96 is a highly potent, selective and non-ATP-competitiveAurora B(IC₅₀=0.316 nM) inhibitor and shows >2000 fold selectivity againstFLT3and KIT. SP-96 shows selective growth inhibition in NCI60 screening, incluing MDA-MD-468 (GI₅₀=107 nM). SP-96 can be used for the research of triple negative breastcancer(TNBC)^[1].

SP-96 is a highly potent, selective and non-ATP-competitiveAurora B(IC₅₀=0.316 nM) inhibitor and shows >2000 fold selectivity against FLT3 (IC₅₀=1475.6 nM) and KIT (IC₅₀=1307.6 nM)^[1].
SP-96 (0-1 μM; 24 hours) is not promiscuous, rather selective for a few cell lines, it inhibits MDA-MB-468, CCRF-CEM, COLO 205 and A498 cell growth with GI₅₀values of 107 nM,47.4 nM, 50.3 nM and 53.2 nM, respectively^[1].
SP-96 (63.2 nM) inhibits Aurora B activity in H460 cells by the characteristics of increased DNA content, and it increases cell volume with enormous nucleus^[1].
SP-96 (0-2 μM) inhibits Aurora B enzymatic activity with an IC₅₀of 0.316 nM and inhibits Aurora A with observed IC₅₀value of 18.975 nM. SP-96 shows >2000 fold selectivity against FLT3 (IC₅₀=1475.6 nM) and KIT (IC₅₀=1307.6 nM). Meanwhile, it exhibits inhibitory effects on other receptor tyrosine kinases (RTKs) namely EGFR, RET and HER2 with IC₅₀value ≥2 μM^[1].

453.47

Solid

C₂₅H₂₀FN₇O

2682114-54-9

Room temperature in continental US; may vary elsewhere.

DMSO : 100 mg/mL(220.52 mM;Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMSO    90%corn oil

  Solubility: ≥ 2.5 mg/mL (5.51 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (5.51 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。