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Deoxycytidine triphosphate trisodium salt
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Deoxycytidine triphosphate trisodium salt图片
CAS NO:109909-44-6

dCTP trisodium salt
2′-Deoxycytidine-5′-triphosphate trisodium salt
Deoxycytidine triphosphate trisodium salt (dCTP trisodium salt) 是可用于 DNA 合成 (DNA synthesis) 的核苷三磷酸。Deoxycytidine triphosphate trisodium salt 可用于实时 PCR,cDNA 合成 和 DNA 测序等研究。
生物活性

Deoxycytidine triphosphate trisodium salt (dCTP trisodium salt) is a nucleoside triphosphate that can be used forDNA synthesis. Deoxycytidine triphosphate trisodium salt has many applications, such asreal-time PCR, cDNA synthesis, and DNA sequencing[1][2][3].

IC50& Target

Human Endogenous Metabolite

 

体外研究
(In Vitro)

Assays of thirteen cell lines, derived from mouse lymphomas, myelomas, myeloid tumors, and a mastocytoma, for sensitivity to growth inhibition by 1-β-D-arabinofuranosylcytosine (ara-C) revealed a spectrum between the most and least sensitive which differed 100-fold from each other. An inverse correlation between sensitivity and cellular Deoxycytidine triphosphate (2′-Deoxycytidine-5′-triphosphate, dCTP) content is found, and this suggested that sensitivity of cells might be increased if the dCTP content was lowered during cell exposure to ara-C[1].
Deoxycytidine triphosphate trisodium salt is a phosphoethanolamine cytidylyltransferase substrate[2].

分子量

533.10

性状

Solid

Formula

C9H13N3Na3O13P3

CAS 号

109909-44-6

中文名称

脱氧胞苷三磷酸三钠盐;2′-脱氧胞苷-5′-三磷酸三钠盐

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O : 250 mg/mL(468.96 mM;Need ultrasonic)

DMSO :< 1 mg/mL (ultrasonic;warming;heat to 80℃)(insoluble or slightly soluble)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.8758 mL9.3791 mL18.7582 mL
5 mM0.3752 mL1.8758 mL3.7516 mL
10 mM0.1876 mL0.9379 mL1.8758 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 100 mg/mL (187.58 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。
 
 
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