(rel)-Oxaliplatin 是一种 DNA 合成抑制剂。(rel)-Oxaliplatin 引起 DNA 交联损伤，阻止 DNA 复制和转录，并诱导细胞凋亡。(rel)-Oxaliplatin 可用于癌症研究。
| 生物活性 | (rel)-Oxaliplatin is aDNA synthesisinhibitor. (rel)-Oxaliplatin causes DNA crosslinking damage, prevents DNA replication and transcription and inducesapoptosis. (rel)-Oxaliplatin can be used forcancerresearch[1][2][3]. |
体外研究
(In Vitro) | (rel)-Oxaliplatin (24-72 hours; 2-128 μM; HCC, HCCLM3 and Hep3B cells) inhibits cell growth and induces apoptosis[1].
(rel)-Oxaliplatin (10 μM; 15-240 mins; CEM cells ) induces primary and secondary DNA lesions, including DNA cross-links (ISC) and DNA-protein cross-links (DPC)[2].
(rel)-Oxaliplatin (0.01 to 100 μM; 24 hours) potently inhibits bladder carcinoma cell lines RT4 and TCCSUP, ovarian carcinoma cell line A2780, colon carcinoma cell line HT-29, glioblastoma cell lines U-373MG and U-87MG, and melanoma cell lines SK-MEL-2 and HT-144 with IC50of 11 μM, 15 μM, 0.17 μM, 0.97 μM, 2.95 μM, 17.6 μM, 30.9 μM and 7.85 μM, respectively[3]
Cell Viability Assay[1] | Cell Line: | HCC, HCCLM3 and Hep3B cells | | Concentration: | 24, 48 and 72 hours | | Incubation Time: | 2, 4, 8, 16, 32, 64 and 128 μM | | Result: | Decreased cell viability in a dose- and time-dependent manner. |
Western Blot Analysis[1] | Cell Line: | HCCLM3 and Hep3B cells | | Concentration: | 48 hours | | Incubation Time: | 10 μM | | Result: | Down-regulated the expression of Bcl-2 and Bcl-xL, and increased the expression of Bax. |
Cell Cycle Analysis[1] | Cell Line: | HCCLM3 and Hep3B cells | | Concentration: | 24 hours | | Incubation Time: | 10 μM | | Result: | Increased the percentage of apoptotic cells (17.70% for HCCLM3 cells; 21.19% for Hep3B cells). |
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体内研究
(In Vivo) | (rel)-Oxaliplatin (5-10 mg/kg; i.p.; for 32 days; nude mice) inhibits tumor growth[1].
| Animal Model: | Nude mice[1] | | Dosage: | 5 and 10 mg/kg | | Administration: | Intraperitoneal injection; for 32 days | | Result: | Reduced tumor volume in HCCLM3 tumor xenografts. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
