Fludarabine triphosphate (F-ara-ATP), the active metabolite ofFludarabine(HY-B0069), is a potent, noncompetitive and specific inhibitor ofDNA primase, with anIC₅₀of 2.3 μM and aK_iof 6.1 μM. Fludarabine triphosphate inhibitsDNA synthesisby blocking DNA primase and primer RNA formation. Fludarabine triphosphate inhibits ribonucleotide reductase and DNA polymerase and ultimately leads to cellularapoptosis^[1][2].

IC50: 2.3 ± 0.3 μM (DNA primase); Ki: 6.1 ± 0.3 μM (DNA primase)^[1]

Fludarabine triphosphate is a more potent inhibitor of the polydeoxythymidylate primase activity than of the DNA polymerase α/δ activities present in the supernatants of CCRF-CEM cells^[1].
Fludarabine triphosphate (10-50 μM) inhibits the incorporation of ATP into primer RNA and dTTP into DNA to a similar extent^[1].

525.17

C₁₀H₁₅FN₅O₁₃P₃

74832-57-8

三磷酸氟达拉滨

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.