NCC-149 是一种选择性的HDAC8抑制剂，可用于神经分化研究。
| 生物活性 | NCC-149 is a selectiveHDAC8inhibitor and can be used for neural differentiation research[1]. |
| IC50& Target | |
体外研究
(In Vitro) | NCC-149 (5 μM; 3 days) remarkably reduces NeuN expression levels in P19 cells[1].
NCC-149 (0-40 μM; 4 days) reduces embryoid body size in a dose-dependent manner[1].
NCC-149 (5 and 20 μM; 24 h) downregulates P19 cell proliferation[1].
NCC-149 (2.5 and 5 μM; 24 h) leads to cell growth retardation by G2/M phase arrest[1].
Western Blot Analysis[1] | Cell Line: | P19 cells | | Concentration: | 5 μM | | Incubation Time: | 3 days | | Result: | Remarkably reduced NeuN expression levels. |
Cell Proliferation Assay[1] | Cell Line: | P19 cells | | Concentration: | 5 and 20 μM | | Incubation Time: | 24 h | | Result: | Downregulated cell proliferation. |
Cell Cycle Analysis[1] | Cell Line: | P19 cells | | Concentration: | 2.5 and 5 μM | | Incubation Time: | 24 h | | Result: | Led to a significant increase in G2/M phase cells, with a slight decrease in S phase cells. |
RT-PCR[1] | Cell Line: | P19 cells | | Concentration: | 25 μM or 2.5 and 5 μM | | Incubation Time: | 4 days (25 μM) or 48 h (2.5 and 5 μM) | | Result: | Did not reduce the HDAC8 expression at the mRNA level.
Significantly and partially reduced cyclin B1 and cyclin A2 gene expression, respectively. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
