WIKI4

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WIKI4

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

838818-26-1

WIKI4 是一种有效的tankyrase抑制剂，其对TNKS2的IC₅₀值为 26 nM。WIKI4 有效抑制Wnt/β-catenin信号传导，其EC₅₀值为 75 nM。WIKI4 通过抑制TNKS2的酶活性来介导其对Wnt/β-catenin信号传导的影响。WIKI4 对 SCLC 细胞具有细胞毒性，其IC₅₀值为 0.02 μM。

生物活性

WIKI4 is a potenttankyraseinhibitor with anIC₅₀of 26 nM forTNKS2. WIKI4 potently inhibitsWnt/β-cateninsignaling and that its half-maximal response dose is 75 nM. WIKI4 mediates its effects onWnt/β-cateninsignaling by inhibiting the enzymatic activity ofTNKS2^[1]^[2]. WIKI4 is cytotoxic to SCLC cells with anIC₅₀value of 0.02 μM^[3].

IC₅₀& Target^[2]

TNKS2

26 nM (IC₅₀)

体外研究
(In Vitro)

WIKI4 (100 nM, 1 μM; 6 days; DLD1 cells) inhibits growth of DLD1 cells relative to DMSO controls in media containing low serum. WIKI4 inhibits expression of β-catenin target genes and cellular responses to Wnt/β-catenin signaling^[1].
WIKI4 (1 μM; 2 hours, 4 hours, 6 hours, or 24 hours; DLD1 cells) significantly increases the steady-state abundance of AXIN1 and AXIN2^[1].

Cell Viability Assay^[1]

Cell Line:	DLD1 cells
Concentration:	100 nM, 1 μM
Incubation Time:	6 days
Result:	Inhibited growth of DLD1 cells.

Western Blot Analysis^[1]

Cell Line:	DLD1 cells
Concentration:	1 μM
Incubation Time:	2 hours,4 hours,6 hours, or 24 hours
Result:	Significantly increased the steady-state abundance of AXIN1 and AXIN2.

分子量

521.59

性状

Solid

Formula

C₂₉H₂₃N₅O₃S

CAS 号

838818-26-1

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 6.8 mg/mL(13.04 mM;Need ultrasonic and warming)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	1.9172 mL	9.5861 mL	19.1721 mL
5 mM	0.3834 mL	1.9172 mL	3.8344 mL
10 mM	0.1917 mL	0.9586 mL	1.9172 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。